Structural Formula Vector Image
Title: 1-Anilino-8-naphthalenesulfonate
CAS Registry Number: 82-76-8
CAS Name: 8-(Phenylamino)-1-naphthalenesulfonic acid
Additional Names: 8-anilino-1-naphthalenesulfonic acid; ANS; phenylperi acid
Molecular Formula: C16H13NO3S
Molecular Weight: 299.34
Percent Composition: C 64.20%, H 4.38%, N 4.68%, O 16.03%, S 10.71%
Literature References: Hydrophobic fluorescent probe for protein studies; originally used in prepn of aniline dyes. Prepd from 8-aminonaphthalenesulfonic acid, aniline and anilinium hydrochloride. Fluorescence spectrum: L. Stryer, Science 162, 526 (1968). Binding to bovine serum albumin: G. Weber, L. B. Young, J. Biol. Chem. 239, 1415 (1964); to lipoproteins: R. A. Muesing, T. Nishida, Biochemistry 10, 2952 (1971); to chymotrypsin: J. D. Johnson et al., ibid. 18, 1292 (1979); L. D. Weber et al., ibid. 1297. Interaction with rod outer segment membrane: U. P. Andley, B. Chakrabarti, ibid. 20, 1687 (1981).
Derivative Type: Magnesium salt
Molecular Formula: C32H24MgN2O6S2
Molecular Weight: 620.98
Percent Composition: C 61.89%, H 3.90%, Mg 3.91%, N 4.51%, O 15.46%, S 10.33%
Properties: Green crystals from water. uv max: 350 nm (e 4.95 ´ 103).
Absorption maximum: uv max: 350 nm (e 4.95 ´ 103)
Derivative Type: Ammonium salt
Molecular Formula: C16H16N2O3S
Molecular Weight: 316.37
Percent Composition: C 60.74%, H 5.10%, N 8.85%, O 15.17%, S 10.14%
Properties: mp 242-244°.
Melting point: mp 242-244°
Use: Fluorescent probe. In protein conformation studies. Magnesium salt as visualization reagent for proteins.

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