Dihydroxyacetone
(dye'' hye drox'' ee as' e tone).
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C3H6O3 90.08

1,3-Dihydroxy-2-propanone. [96-26-4].
» Dihydroxyacetone contains not less than 98.0 percent and not more than 102.0 percent of C3H6O3, calculated on the anhydrous basis.
Packaging and storage— Preserve in tight containers in a cool place.
USP Reference standards 11
USP Dihydroxyacetone RS Click to View Structure
Identification—
B: The RF value of the principal spot in the chromatogram of the Test solution corresponds to that of the Standard preparation as obtained in the Chromatographic purity test.
pH 791: between 4.0 and 6.0, in a solution (1 in 20).
Water, Method I 921: not more than 0.2%.
Residue on ignition 281: not more than 0.1%.
Limit of iron 241: not more than 0.002%.
Chromatographic purity—
Adsorbent: 0.25-mm layer of chromatographic silica gel mixture.
Glycerin solution— Dilute an accurately measured volume of glycerin with methanol to obtain a solution having a concentration of about 0.25 mg per mL.
Test solution— Dissolve an accurately weighed quantity of Dihydroxyacetone in methanol to obtain a solution containing about 50 mg per mL.
Standard solution— Dissolve an accurately weighed quantity of USP Dihydroxyacetone RS in methanol, and mix to obtain a solution having a known concentration of about 50 mg per mL.
Application volume: 1 µL.
Developing solvent system: a mixture of acetone and water (19:1).
Procedure— Proceed as directed for Thin-Layer Chromatography under Chromatography 621. Spray the plate with a mixture of toluene, a saturated solution of lead tetraacetate in glacial acetic acid, and a 1% solution of dichlorofluorescein in alcohol (190:5:1), and dry the plate for 5 minutes at 105. Examine the plate under short-wavelength UV light, and compare the intensities of the glycerin spot observed in the chromatogram of the Test solution with that of the principal spot in the chromatogram of the Glycerin solution: the glycerin spot from the chromatogram of the Test solution is not larger or more intense than the principal spot obtained from the Glycerin solution (0.5%), and no other secondary spots are observed in the chromatogram of the Test solution.
Limit of protein— Dissolve 25 g of Dihydroxyacetone in 100 mL of water. Transfer 100 µL of this solution to a 5-mL volumetric flask, dilute with brilliant blue G TS to volume, and mix. Determine the absorbance of this solution at about 595 nm with a suitable spectrophotometer, using 100 µL of water and 5 mL of brilliant blue G TS as the blank: the absorbance of the test solution is not more than 0.400.
Assay— Dissolve an accurately weighed quantity of about 0.1 g of Dihydroxyacetone in 20 mL of water, add 20 mL of 0.1 M periodic acid, and allow to stand at room temperature in the dark for 20 minutes. Add about 3 g of sodium bicarbonate, 20 mL of 0.6 M potassium iodide, and 3 mL of starch TS, and titrate with 0.05 M sodium arsenite VS. Perform a blank titration, and make any necessary correction. Each mL of 0.05 M sodium arsenite is equivalent to 4.504 mg of C3H6O3.
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