» Chloramphenicol Tablets contain not less than 90.0 percent and not more than 120.0 percent of the labeled amount of C11H12Cl2N2O5.
Packaging and storage Preserve in tight containers.
Labeling Label Tablets to indicate that they are for veterinary use only and are not to be used in animals raised for food production.
Identification The retention time of the major peak in the chromatogram of the Assay preparation corresponds to that in the chromatogram of the Standard preparation as obtained in the Assay.
Disintegration 701: 60 minutes.
Uniformity of dosage units 905: meet the requirements.
Standard preparation Transfer about 25 mg of USP Chloramphenicol RS, accurately weighed, to a 200-mL volumetric flask, add 10 mL of water, and heat on a steam bath until completely dissolved. Cool to room temperature, dilute with Mobile phase to volume, and mix. Filter a portion of this solution through a 0.5-µm or finer porosity filter, and use the clear filtrate as the Standard preparation.
Assay preparation Weigh and finely powder not less than 20 Tablets. Transfer an accurately weighed portion of the powder, equivalent to about 500 mg of chloramphenicol, to a 200-mL volumetric flask, add 80 mL of water, and heat on a steam bath for 20 minutes, with occasional mixing. Cool to room temperature, dilute with water to volume, and mix. Transfer 5.0 mL of the resulting solution to a 100-mL volumetric flask, dilute with Mobile phase to volume, and mix. Filter a portion of this solution through a 0.5-µm or finer porosity filter, and use the clear filtrate as the Assay preparation.
Auxiliary Information Please check for your question in the FAQs before contacting USP.Chromatographic Column
USP32NF27 Page 1888
Chromatographic columns text is not derived from, and not part of, USP 32 or NF 27.