Trypan Blue
Structural Formula Vector Image
Title: Trypan Blue
CAS Registry Number: 72-57-1
CAS Name: 3,3¢-[(3,3¢-Dimethyl[1,1¢-biphenyl]-4,4¢-diyl)bis(azo)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonic acid] tetrasodium salt
Additional Names: C.I. Direct Blue 14; C.I. 23850; 3,3¢-[(3,3¢-dimethyl-4,4¢-biphenylene)bis(azo)]bis(5-amino-4-hydroxy-2,7-naphthalenedisulfonic acid) tetrasodium salt; tetrasodium 3,3¢-[(3,3¢-dimethyl-4,4¢-biphenylene)bis(azo)]bis(5-amino-4-hydroxy-2,7-naphthalenedisulfonate); sodium ditolyl-diazobis-8-amino-1-naphthol-3,6-disulfonate; Benzamine Blue; Diamine Blue; Benzo Blue; Congo Blue; Dianil Blue; Naphthylamine Blue; Niagara Blue
Molecular Formula: C34H24N6Na4O14S4
Molecular Weight: 960.81
Percent Composition: C 42.50%, H 2.52%, N 8.75%, Na 9.57%, O 23.31%, S 13.35%
Literature References: Prepd by coupling diazotized o-tolidine with 5-amino-4-hydroxy-2,7-naphthalenedisulfonic acid in sodium carbonate soln: Lewers, Lowy, Ind. Eng. Chem. 17, 1289-1290 (1925); Colour Index vol. 4 (3rd ed., 1971) p 4198. Toxicity study: Anderson et al., Proc. Soc. Exp. Biol. Med. 31, 825 (1934). Review of teratogenicity studies: R. L. Cahen, Clin. Pharmacol. Ther. 5, 480 (1964).
Properties: Bluish-gray powder. Sol in water forming a deep blue soln with violet tinge. Almost insol in alcohol. LD100 i.v. in rats: 300 mg/kg (Anderson).
Toxicity data: LD100 i.v. in rats: 300 mg/kg (Anderson)
Use: Biological stain.

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