Fully Hydrogenated Rapeseed Oil
Fully hydrogenated rapeseed oil [84681-71-0]. DEFINITION
Fully Hydrogenated Rapeseed Oil is the product obtained by refining and hydrogenating oil obtained from the seeds of Brassica napus and Brassica campestris (Fam. Cruciferae). The product is a mixture of triglycerides in which the fatty acid composition is a mixture of saturated fatty acids.
IDENTIFICATION
• A.
It meets the requirements of the test for Fats and Fixed Oils, Fatty Acid Composition 401.
IMPURITIES
• Alkaline Impurities
Sample solution:
Prepare a mixture of 2.0 g of Fully Hydrogenated Rapeseed Oil, 1.5 mL of alcohol, and 3.0 mL of toluene. Dissolve by gentle heating.
Analysis:
To the Sample solution add 0.05 mL of bromophenol blue TS, and titrate with 0.01 N hydrochloric acid to a yellow endpoint.
Acceptance criteria:
NMT 0.4 mL of 0.01 N hydrochloric acid is required.
• Residue on Ignition 281:
NMT 0.5%, when a 5-g sample of Fully Hydrogenated Rapeseed Oil is ignited at an ignition temperature of 800 ± 25
• Heavy Metals, Method II 231:
NMT 10 ppm
• Limit of Nickel
Sample solution:
Weigh 5.0 g of Fully Hydrogenated Rapeseed Oil into a previously tared platinum or silica crucible. Cautiously heat the substance, and introduce into it a wick formed from twisted ashless filter paper. Ignite the wick. When the substance ignites, stop heating. After combustion, ignite in a muffle furnace at about 600. Continue the incineration until white ash is obtained. After cooling, transfer the residue, with the aid of two 2-mL portions of diluted hydrochloric acid, to a 25-mL volumetric flask. Add 0.3 mL of nitric acid, and dilute with water to volume.
Standard stock solution:
0.2 µg/mL of nickel prepared from nickel standard solution TS and water. Prepare immediately before use.
Standard solutions:
Into three identical 10-mL volumetric flasks introduce 1.0, 2.0, and 4.0 mL of Standard stock solution, respectively. To each flask add a 2.0-mL portion of the Sample solution, and dilute with water to volume.
Instrumental conditions
Mode:
Atomic absorption spectrophotometer equipped with a graphite furnace
Absorbance:
232.0 nm
Lamp:
Nickel hollow-cathode
Analysis
Samples:
Sample solution and Standard solutions
Concomitantly determine the absorbances of the Samples at least three times each. Record the average of the steady readings for each of the Standard solutions and the Sample solution. Plot the absorbances of the Standard solutions and the Sample solution versus the added quantity of nickel. [NoteThe Sample solution should be plotted as if it had a content of added nickel equivalent to 0 µg. ] Extrapolate the line joining the points on the graph until it meets the concentration axis. The distance between this point and the intersection of the axes represents the concentration of nickel, C, in µg/mL, in the Sample solution.
Calculate the content of nickel in the portion of Fully Hydrogenated Rapeseed Oil taken:
Result = (C/W) × V
Acceptance criteria:
NMT 1 ppm
• Limit of Erucic Acid:
NMT 1.0%, as determined under Specific Tests, in the test Fats and Fixed Oils, Fatty Acid Composition 401
SPECIFIC TESTS
• Fats and Fixed Oils, Fatty Acid Composition 401:
Fully Hydrogenated Rapeseed Oil exhibits the fatty acid composition profile shown in Table 1.
Table 1
• Fats and Fixed Oils, Acid Value 401:
NMT 6.0
• Fats and Fixed Oils, Peroxide Value 401:
NMT 2.0
• Fats and Fixed Oils, Unsaponifiable Matter 401:
NMT 1.5%
ADDITIONAL REQUIREMENTS
• Packaging and Storage:
Preserve in tight, light-resistant containers. No storage requirements specified.
Auxiliary Information
Please check for your question in the FAQs before contacting USP.
USP35NF30 Page 1941
Pharmacopeial Forum: Volume No. 32(6) Page 1771
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