Garlic Fluidextract is prepared as follows. Soak 1000 g of Garlic, whole or sliced, in a volume of a mixture of water and alcohol (between 80:20 and 50:50) sufficient to cover the cloves. Store in a suitable container for a length of time sufficient to extract the constituents, avoiding any contamination, and then filter. Concentrate the filtrate, if necessary, at the lowest possible temperature, and add sufficient water or alcohol to make the product measure 1000 mL. [NoteComplete extraction may require 30 days. ]
• A. Thin-Layer Chromatographic Identification Test
Extraction column: Use a solid-phase extraction column that contains benzenesulfonylpropyl bonded to silica gel in the hydrogen form having a sorbent mass-to-column volume ratio of 1 g per 6 mL, or equivalent. Condition the column before use by washing with 10 mL of methanol and 10 mL of water. [NoteDo not allow the column to dry. ]
Standard solution: 0.5 mg/mL of USP S-Allyl-l-cysteine RS in a mixture of methanol and water (1:1)
Sample solution: Mix 1 mL of Fluidextract and 5 mL of water, and transfer to the Extraction column. Allow to drain, and discard the eluate. Wash the column with 10 mL of water and 10 mL of methanol, discarding the eluates. Elute the amino acid fraction with 3 mL of ammonium hydroxide solution in methanol (7 in 100), and collect the eluate.
Adsorbent: 0.25-mm layer of chromatographic silica gel, typically 20 cm long (TLC plates)
Application volume: 5 µL
Developing solvent system: Ethyl acetate, methanol, acetone, glacial acetic acid, and water (10:4:3:1:3)
Spray reagent: Iodoplatinate TS
Samples: Standard solution and Sample solution
Develop the chromatograms until the solvent front has moved up about three-fourths of the plate, in a saturated chamber. Remove the plate, and allow the solvent to evaporate. Spray with the Spray reagent, and examine the plate.
Acceptance criteria: The chromatogram of the Sample solution exhibits, among several yellow spots on the purple plate, a yellow spot at an RF value of about 0.4 corresponding to that of the yellow spot obtained in the chromatogram of the Standard solution (presence of S-allyl-l-cysteine).
• Content of S-Allyl-l-cysteine
Mobile phase: Transfer 15.8 g of sodium citrate dihydrate to 250 mL of water, and carefully add 10.5 mL of hydrochloric acid. Using a pH meter, adjust with 6 N sodium hydroxide to a pH of 4.0. Dilute with water to 1000 mL, and mix.
Derivatizing reagent: Dissolve 0.8 g of o-phthalaldehyde in 2 mL of 2-mercaptoethanol. Add to a solution containing 24.70 g of boric acid and 22.35 g of potassium hydroxide in 1000 mL of water, and mix.
Reactivating solution: 0.2 N sodium hydroxide. Prepare by dissolving 0.8 g of sodium hydroxide in 100 mL of water.
Standard solution: 0.01 mg/mL of USP S-Allyl-l-cysteine RS in water
Sample solution: Transfer about 2.0 g of Fluidextract, accurately weighed, to a 100-mL volumetric flask, dilute with trichloroacetic acid solution (5 in 100) to volume, and mix. Centrifuge for 5 min, and filter the supernatant.
Detector: Fluorometric detector; excitation wavelength of 340 nm and emission wavelength of 455 nm
Column: 4.6-mm × 12-cm; packing L17
Column temperature: 40
Injection size: 10 µL
[NoteThe Mobile phase and the Reactivating solution are pumped separately, each at the rate of 0.4 mL/min, by pumps connected to the opposing arms of a tee. The outlet of the tee is connected to the injector and the chromatographic column. The outlet of the column is attached to a tee, the opposing arm of which is attached to a tube from which the Derivatizing reagent is constantly pumped through the system at a rate of 0.6 mL/min. The outlet of the tee is connected to a 0.5-mm × 2.0-m postcolumn polytef reaction coil maintained at 40. The outlet of the reaction coil is connected to the detector. The system is programmed to deliver the Mobile phase for 10 min, the Reactivating solution for the next 6 min, and the Mobile phase for the 24 min remaining before the next injection. ]
Sample: Standard solution
Capacity factor (k¢): 2.54.5
Tailing factor: NMT 2.0 for the S-allyl-l-cysteine peak
Relative standard deviation: NMT 2.0% for the S-allyl-l-cysteine peak in repeated injections
Samples: Standard solution and Sample solution
Calculate the percentage of S-allyl-l-cysteine (C6H11SN) in the portion of Fluidextract taken:
Result = (rU/rS) × CS × (V/W) × 100
Acceptance criteria: NLT 0.05% on the dried basis
• Heavy Metals, Method II 231: NMT 10 ppm
• Microbial Enumeration Tests 2021: The total aerobic bacterial count does not exceed 103 cfu/mL, and the total combined molds and yeasts count does not exceed 102 cfu/mL.
• Absence of Specified Microorganisms 2022: Meets the requirements of the tests for absence of Salmonella species and Escherichia coli
• Residue on Evaporation: Proceed as directed under Botanical Extracts 565: NLT 20% of the Fluidextract portion taken remains as residue.
• Articles of Botanical Origin, Total Ash 561: NMT 3.0%
• pH 791: 4.56.5
• Other Requirements: Meets the requirements under Botanical Extracts 565, General Pharmacopeial Requirements, sections for Packaging and Storage, Labeling, Pesticide Residues, and Alcohol Content for Fluidextracts
• USP Reference Standards 11
USP S-Allyl-l-cysteine RS
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USP35NF30 Page 1314