Cupric Chloride
CuCl2·2H2O 170.48
CuCl2 134.45
Copper chloride (CuCl2) dihydrate;
Copper(2+) chloride dihydrate
[10125-13-0].
Anhydrous[7447-39-4].
(kue' prik klor' ide).
CuCl2·2H2O 170.48
CuCl2 134.45
Copper chloride (CuCl2) dihydrate;
Copper(2+) chloride dihydrate
[10125-13-0].Anhydrous
[7447-39-4].DEFINITION
Cupric Chloride contains NLT 99.0% and NMT 100.5% of CuCl2, calculated on the dried basis.
IDENTIFICATION
• A. Identification Tests—General, Chloride 
191
:
A solution (1 in 20) meets the requirements.
191:
A solution (1 in 20) meets the requirements.
• B. Identification Tests—General, Copper 191:
A solution (1 in 20) meets the requirements.
191:
A solution (1 in 20) meets the requirements.
ASSAY
• Procedure
Sample solution:
8 mg/mL of Cupric Chloride in water
Analysis:
To the Sample solution add 4 mL of acetic acid and 3 g of potassium iodide. Titrate the liberated iodine with 0.1 N sodium thiosulfate VS, adding 2 g of potassium thiocyanate and 3 mL of starch TS as the endpoint is approached. Each mL of 0.1 N sodium thiosulfate is equivalent to 13.45 mg of Cupric Chloride (CuCl2).
Acceptance criteria:
99.0%–100.5% on the dried basis
IMPURITIES
• Limit of Sodium
Sample stock solution:
Transfer 10.0 g of Cupric Chloride to a 100-mL volumetric flask, add water, and swirl to dissolve. Add 5 mL of nitric acid, and dilute with water to volume. This solution contains 0.1 g/mL of cupric chloride.
Sample solutions:
To three 25-mL volumetric flasks add a volume of the Sample stock solution equivalent to the Sample Weight given in Table 1. To two of the flasks add the amounts of reference analyte ion specified in Table 1. Add 2 mL of potassium chloride solution (1 in 20 ) to each flask, and dilute with water to volume.
Analysis:
Using atomic absorption spectrophotometry (see Spectrophotometry and Light-Scattering 851), analyze the Sample solutions by the method of standard addition analysis according to Table 1.
851), analyze the Sample solutions by the method of standard addition analysis according to Table 1.
Acceptance criteria:
NMT 0.02%
Table 1
| Limit Test | Wave- length (nm) |
Sample Weight (g) |
Reference Ion Added (mg) |
Flame Type | Background Correction |
|---|---|---|---|---|---|
| Sodium | 589.0 | 0.1 | 0.01/0.02 | Air– acetylene |
No |
| Potassium | 766.5 | 0.1 | 0.01/0.02 | Air– acetylene |
No |
| Calcium | 422.7 | 2.0 | 0.05/0.10 | Air– acetylene |
No |
| Iron | 248.3 | 2.0 | 0.05/0.10 | Air– acetylene |
Yes |
| Nickel | 232.0 | 2.0 | 0.10/0.20 | Air– acetylene |
No |
• Limit of Potassium
Sample stock solution:
Prepare as directed in the test for Limit of Sodium.
Sample solutions:
To three 25-mL volumetric flasks add a volume of the Sample stock solution equivalent to the Sample Weight given in Table 1. To two of the flasks add the amounts of reference analyte ion specified in Table 1, and dilute with water to volume.
Analysis:
Using atomic absorption spectrophotometry (see Spectrophotometry and Light-Scattering 851), analyze the Sample solutions by the method of standard addition analysis according to Table 1.
851), analyze the Sample solutions by the method of standard addition analysis according to Table 1.
Acceptance criteria:
NMT 0.01%
• Limit of Calcium
Sample stock solution:
Prepare as directed in the test for Limit of Sodium.
Sample solutions:
To three 25-mL volumetric flasks add a volume of the Sample stock solution equivalent to the Sample Weight given in Table 1. To two of the flasks add the amounts of reference analyte ion specified in Table 1, and dilute with water to volume.
Analysis:
Using atomic absorption spectrophotometry (see Spectrophotometry and Light-Scattering 851), analyze the Sample solutions by the method of standard addition analysis according to Table 1.
851), analyze the Sample solutions by the method of standard addition analysis according to Table 1.
Acceptance criteria:
NMT 0.005%
• Limit of Iron
Sample stock solution:
Prepare as directed in the test for Limit of Sodium.
Sample solutions:
To three 25-mL volumetric flasks add a volume of the Sample stock solution equivalent to the Sample Weight given in Table 1. To two of the flasks add the amounts of reference analyte ion specified in Table 1, and dilute with water to volume.
Analysis:
Using atomic absorption spectrophotometry (see Spectrophotometry and Light-Scattering 851), analyze the Sample solutions by the method of standard addition analysis according to Table 1.
851), analyze the Sample solutions by the method of standard addition analysis according to Table 1.
Acceptance criteria:
NMT 0.005%
• Limit of Nickel
Sample stock solution:
Prepare as directed in the test for Limit of Sodium.
Sample solutions:
To three 25-mL volumetric flasks add a volume of the Sample stock solution equivalent to the Sample Weight given in Table 1. To two of the flasks add the amounts of reference analyte ion specified in Table 1, and dilute with water to volume.
Analysis:
Using atomic absorption spectrophotometry (see Spectrophotometry and Light-Scattering 851), analyze the Sample solutions by the method of standard addition analysis according to Table 1.
851), analyze the Sample solutions by the method of standard addition analysis according to Table 1.
Acceptance criteria:
NMT 0.01%
• Insoluble Matter
Sample:
10 g of Cupric Chloride
Analysis:
Transfer the Sample to a 250-mL beaker. Add 100 mL of water and 2 mL of hydrochloric acid. Cover the beaker, and heat to boiling. Digest the hot solution on a steam bath for 1 h, and pass through a tared, filtering crucible of fine-pore size. Rinse the beaker with hot water, passing the rinsings through the filter, and finally wash the filter with additional hot water. Retain the combined filtrate and washings for the test for Limit of Sulfate. Dry the filter at 105
.
.
Acceptance criteria:
The residue weighs NMT 1.0 mg (0.01%).
• Limit of Sulfate
Analysis:
Heat to boiling the combined filtrate and washings retained from the test for Insoluble Matter. Add 10 mL of barium chloride TS, digest for 2 h on a steam bath, and allow to stand overnight. Pass the solution through a tared, porcelain filtering crucible of medium-pore size, and wash the residue with two 10-mL portions of hot water. Ignite at 800 ± 25 to constant weight.
to constant weight.
Acceptance criteria:
The weight of the residue, corrected for the weight obtained in a blank test, does not exceed 1.2 mg (0.005%).
SPECIFIC TESTS
• Loss on Drying 731:
Dry a sample at 105 for 16 h: it loses 20.9%-21.4% of its weight.
731:
Dry a sample at 105 for 16 h: it loses 20.9%-21.4% of its weight.
ADDITIONAL REQUIREMENTS
• Packaging and Storage:
Preserve in tight containers. Store at controlled room temperature.
Auxiliary Information—
Please check for your question in the FAQs before contacting USP.
| Topic/Question | Contact | Expert Committee |
|---|---|---|
| Monograph | Behnam Davani, Ph.D., M.B.A.
Senior Scientific Liaison 1-301-816-8394 |
(SM12010) Monographs - Small Molecules 1 |
USP35–NF30 Page 2780
Pharmacopeial Forum: Volume No. 29(6) Page 1864