Aromatic Castor Oil
Aromatic Castor Oil is Castor Oil containing suitable flavoring agents. It contains NLT 95.0% of castor oil.
Internal standard solution: 12 mg/mL of di(2-ethylhexyl)phthalate in chloroform
Standard solution: Transfer 100 mg of Castor Oil to a 100-mL boiling flask equipped with a suitable reflux condenser connected by a ground-glass joint. Add 30 mL of a mixture of 300 mL of methanol and 3.7 mL of sulfuric acid, reflux in a water bath maintained at 7580 for 2.5 h, cool, and rinse down the condenser with 10 mL of water. Transfer the contents of the flask to a 125-mL separator with the aid of 10 mL of water. Rinse the condenser and the flask with 25 mL of solvent hexane, and transfer to the separator. Shake the separator for 2 min, and draw off the aqueous layer into a second 125-mL separator. Add 20 mL of solvent hexane to the second separator, shake for 2 min, discard the aqueous layer, and transfer the solvent hexane layer to the first separator with the aid of 10 mL of solvent hexane. Wash the combined extracts with three 5-mL portions of water, discarding the washings, and transfer the washed extract to a 125-mL conical flask, through a funnel containing anhydrous sodium sulfate, with the aid of 25 mL of solvent hexane. Place the flask in a hot water bath, and evaporate with the aid of a current of air to dryness. To the residue add 10.0 mL of Internal standard solution, and mix until solution is complete.
Sample solution: Transfer an amount of Aromatic Castor Oil, well-shaken and nominally equivalent to 100 mg of castor oil, to a long-neck, round-bottom 100-mL boiling flask equipped with a suitable reflux condenser connected by a ground-glass joint. Proceed as directed for the Standard solution, beginning with Add 30 mL of a mixture of 300 mL of methanol and 3.7 mL of sulfuric acid .
Detector: Flame ionization
Column: 1.8-m × 4-mm column packed with 4% liquid phase G25 on support S1
Column conditioning: Flush with helium for 25 min, then heat without further flushing at 250 for NLT 30 min, then cool to room temperature, and finally heat while helium is flowing through it at 250 for NLT 60 min.
Flow rate: Adjust to obtain a peak due to castor oil 5.5 min after introduction of the specimen and an internal standard peak 8 min after introduction of the specimen.
Carrier gas: Helium
Injection size: 5 µL
Samples: Standard solution and Sample solution
Measure the heights of the peaks due to castor oil and the Internal standard solution.
Calculate the percentage of castor oil in the portion of Aromatic Castor Oil taken:
Result = (RU/RS) × (Ws/WU) × 100
Acceptance criteria: NLT 95.0%
• Alcohol Determination, Method I 611: NMT 4.0% of C2H5OH
• Packaging and Storage: Preserve in tight containers.