Depyrogenate all glassware and other heat-stable materials in a hot air oven using a validated process.1 A commonly used minimum time and temperature is 30 minutes at 250. If employing plastic apparatus, such as microplates and pipet tips for automatic pipetters, use apparatus that is shown to be free of detectable endotoxin and does not interfere in the test. [note—In this chapter, the term “tube” includes any other receptacle such as a microtiter well. ]