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C6H11AuO5S 392.18

Gold, (1-thio-d-glucopyranosato)-.
(1-Thio-d-glucopyranosato)gold [12192-57-3].
» Aurothioglucose contains not less than 95.0 percent and not more than 105.0 percent of C6H11AuO5S, calculated on the dried basis. It is stabilized by the addition of a small amount of Sodium Acetate.
Packaging and storage— Preserve in tight, light-resistant containers. Store at room temperature.
A: Dissolve a suitable quantity in water to obtain a solution containing 4 mg per mL. Apply 10 µL of this solution and 10 µL of an aqueous Standard solution of USP Aurothioglucose RS containing 4 mg per mL to a suitable thin-layer chromatographic glass microfilament sheet (see Chromatography 621) impregnated with silicic acid and a suitable fluorescing substance. Allow the spots to dry, and develop the chromatogram in a solvent system consisting of a mixture of n-propyl alcohol, water, and ethyl acetate (3:3:1) until the solvent front has moved about three-fourths of the length of the plate. Remove the sheet from the developing chamber, mark the solvent front, and allow the solvent to evaporate. Locate the spots on the plate by examination under short-wavelength UV light: the RF value of the principal spot obtained from the solution under test corresponds to that obtained from the Standard solution.
B: To a portion of the filtrate obtained in the Assay add barium chloride TS: a heavy, white precipitate is formed.
Specific rotation 781S: between +65 and +75.
Test solution: 10 mg per mL, in water.
Loss on drying 731 Dry it over phosphorus pentoxide for 24 hours: it loses not more than 1.0% of its weight.
Assay— Accurately weigh about 1 g of Aurothioglucose, and dissolve in 100 mL of water in a 300-mL Kjeldahl flask. Slowly add 10 mL of nitric acid, and when the reaction has subsided, boil the mixture for 5 minutes. Filter, wash well the separated gold with hot water, dry, and ignite to constant weight. The weight of the gold so obtained, multiplied by 1.991, represents the weight of C6H11AuO5S in the portion of Aurothioglucose taken.
Auxiliary Information— Please check for your question in the FAQs before contacting USP.
Topic/Question Contact Expert Committee
Monograph Clydewyn M. Anthony, Ph.D.
(MDCCA05) Monograph Development-Cough Cold and Analgesics
Reference Standards Lili Wang, Technical Services Scientist
USP32–NF27 Page 1607
Pharmacopeial Forum: Volume No. 29(6) Page 1847