d-Streptamine, [O-2-amino-2-deoxy--d-glucopyranosyl-(1®4)-O-[O-2,6-diamino-2,6-dideoxy--l-idopyranosyl-(1®3)--d-ribofuranosyl-(1®5)]-2-deoxy-, sulfate (salt).
O-2,6-Diamino-2,6-dideoxy--l-idopyranosyl-(1®3)-O--d-ribofuranosyl-(1®5)-O-[2-amino-2-deoxy--d-glucopyranosyl-(1®4)]-2-deoxystreptamine sulfate (salt) [1263-89-4].
Base 615.64 [59-04-1; 7542-37-2].
» Paromomycin Sulfate is the sulfate salt of an antibiotic substance or substances produced by the growth of Streptomyces rimosus var. paromomycinus, or a mixture of two or more such salts. It has a potency equivalent to not less than 675 µg of paromomycin (C23H45N5O14) per mg, calculated on the dried basis.
Packaging and storage Preserve in tight containers.
A: Prepare a test solution in water containing 10 mg of paromomycin per mL. Apply 25 µL of this solution and 25 µL of a Standard solution of USP Paromomycin Sulfate RS containing 10 mg of paromomycin per mL to a suitable thin-layer chromatographic plate (see Chromatography 621) coated with a 0.25-mm layer of chromatographic silica gel. Allow the spots to dry, place the plate in a developing chamber, and develop the chromatogram in a solvent system consisting of a mixture of freshly prepared ammonium acetate solution (4 in 100), n-propyl alcohol, and ammonium hydroxide (30:10:6) until the solvent front has moved about three-fourths of the length of the plate. Remove the plate from the chamber, mark the solvent front, and allow it to air-dry for 10 minutes. Heat the plate at 105 for 1 hour, allow to cool, and spray with a solution of ninhydrin in butanol (1 in 100). Heat the plate at 105 for 5 minutes: paromomycin appears as a red spot, and the RF value of the principal spot obtained from the test solution corresponds to that obtained from the Standard solution.
B: It meets the requirements of the tests for Sulfate 191.
Specific rotation 781S: between +50 and +55.
Test solution: 50 mg per mL, in water.
pH 791: between 5.0 and 7.5, in a solution (3 in 100).
Loss on drying 731 Dry about 100 mg in a capillary-stoppered bottle in vacuum at a pressure not exceeding 5 mm of mercury at 60 for 3 hours: it loses not more than 5.0% of its weight.
Residue on ignition 281: not more than 2.0%, the charred residue being moistened with 2 mL of nitric acid and 5 drops of sulfuric acid.
Assay Proceed with Paromomycin Sulfate as directed under AntibioticsMicrobial Assays 81.
Auxiliary Information Please check for your question in the FAQs before contacting USP.
USP32NF27 Page 3213