Mobile phase , Internal standard solution, Standard preparation, and Chromatographic system—Prepare as directed in the Assay under Hydrocortisone Valerate.

Assay preparation— Transfer an accurately weighed quantity of Cream, equivalent to about 1 mg of hydrocortisone valerate, to a screw-capped tube. Add 8.0 mL of a mixture of methanol and water (3:1), and swirl to disperse. Heat at 80 for 1 minute, swirl again, and allow to cool to room temperature. Add 2.0 mL of Internal standard solution, and mix. Centrifuge for 5 minutes, and filter, if necessary, to obtain a clear supernatant.

Procedure— Proceed as directed for Procedure in the Assay under Hydrocortisone Valerate. Calculate the quantity, in mg, of hydrocortisone valerate (C26H38O6), in the portion of Cream taken by the formula: in which the terms are as defined therein.