*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : ST3000000
CHEMICAL NAME : p-Phenylenediamine, 2-nitro-
CAS REGISTRY NUMBER : 5307-14-2
BEILSTEIN REFERENCE NO. : 2210195
REFERENCE : 3-13-00-00271 (Beilstein Handbook Reference)
LAST UPDATED : 199710
DATA ITEMS CITED : 54
MOLECULAR FORMULA : C6-H7-N3-O2
MOLECULAR WEIGHT : 153.16
WISWESSER LINE NOTATION : ZR DZ BNW
COMPOUND DESCRIPTOR : Tumorigen
Mutagen
Reproductive Effector
SYNONYMS/TRADE NAMES :
* 4-Amino-2-nitroaniline
* C.I. 76070
* C.I. Oxidation Base 22
* 1,4-Diamino-2-nitrobenzene
* 1,4-Diaminonitrobenzol
* Durafur Brown 2R
* Dye GS
* Fouramine 2R
* Fourrine 36
* Fourrine Brown 2R
* NCI-C02222
* 2-Nitro-4-aminoaniline
* 2-Nitro-1,4-benzenediamine
* 2-Nitro-1,4-diaminobenzene
* o-Nitro-p-phenylenediamine
* 2-Nitro-1,4-phenylenediamine
* Nitro-p-phenylenediamine
* 2-Nitro-p-phenylenediamine
* Oxidation Base 22
* Ursol Brown RR
* Zoba Brown RR
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 2100 mg/kg
TOXIC EFFECTS :
Kidney, Ureter, Bladder - other changes in urine composition
REFERENCE :
JSCCA5 Journal of the Society of Cosmetic Chemists. (Soc. of Cosmetic
Chemists, 1995 Broadway, Suite 1701, New York, NY 10023) V.1- 1947-
Volume(issue)/page/year: 23,259,1972
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 348 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub.,
1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
Volume(issue)/page/year: 2,657,1977
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 214 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
GPOLA4 Genetica Polonica. (Ars Polona, POB 1001, 00-068 Warsaw 1, Poland)
V.1- 1960- Volume(issue)/page/year: 26,109,1985
** TUMORIGENIC DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 288 gm/kg/78W-C
TOXIC EFFECTS :
Tumorigenic - neoplastic by RTECS criteria
Liver - tumors
REFERENCE :
NCITR* National Cancer Institute Carcinogenesis Technical Report Series.
(Bethesda, MD) No.0-205. For publisher information, see NTPTR*.
Volume(issue)/page/year: NCI-TR-169,1979
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 144 gm/kg/78W-C
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Liver - tumors
REFERENCE :
NCITR* National Cancer Institute Carcinogenesis Technical Report Series.
(Bethesda, MD) No.0-205. For publisher information, see NTPTR*.
Volume(issue)/page/year: NCI-TR-169,1979
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE : 1600 mg/kg
SEX/DURATION : female 6-15 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
e.g., stunted fetus)
Reproductive - Specific Developmental Abnormalities - craniofacial
(including nose and tongue)
Reproductive - Specific Developmental Abnormalities - cardiovascular
(circulatory) system
REFERENCE :
TJADAB Teratology, The International Journal of Abnormal Development. (Alan
R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1968-
Volume(issue)/page/year: 19,37A,1979
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE : 1600 mg/kg
SEX/DURATION : female 6-15 day(s) after conception
TOXIC EFFECTS :
Reproductive - Specific Developmental Abnormalities - craniofacial
(including nose and tongue)
Reproductive - Specific Developmental Abnormalities - musculoskeletal system
REFERENCE :
TJADAB Teratology, The International Journal of Abnormal Development. (Alan
R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1968-
Volume(issue)/page/year: 24,253,1981
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE : 2240 mg/kg
SEX/DURATION : female 6-15 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - post-implantation mortality (e.g. dead and/or
resorbed implants per total number of implants)
Reproductive - Specific Developmental Abnormalities - eye/ear
REFERENCE :
TJADAB Teratology, The International Journal of Abnormal Development. (Alan
R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1968-
Volume(issue)/page/year: 24,253,1981
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE : 1280 mg/kg
SEX/DURATION : female 6-15 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death,
e.g., stunted fetus)
REFERENCE :
TJADAB Teratology, The International Journal of Abnormal Development. (Alan
R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1968-
Volume(issue)/page/year: 24,253,1981
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 5 ug/plate
REFERENCE :
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
V.1- 1869- Volume(issue)/page/year: 255,506,1975
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 3300 ng/plate
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 7(Suppl
5),1,1985
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 333 ug/plate
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 7(Suppl
5),1,1985
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 100 ug/plate
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 7(Suppl
5),1,1985
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 4170 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 272,145,1992
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Human HeLa cell
DOSE/DURATION : 100 nmol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
38,2621,1978
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Human Lymphocyte
DOSE/DURATION : 50 mg/L/24H
REFERENCE :
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
V.1- 1869- Volume(issue)/page/year: 255,506,1975
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 100 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 97,359,1982
TYPE OF TEST : Body fluid assay
TEST SYSTEM : Rodent - rat Bacteria - Salmonella typhimurium
DOSE/DURATION : 200 ug/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 66,241,1979
TYPE OF TEST : Micronucleus test
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 400 mg/kg/24H (Continuous)
REFERENCE :
CYTOAN Cytologia. (Japan Pub. Trading Co. (USA), 1255 Howard St., San
Francisco, CA 94103) V.1- 1929- Volume(issue)/page/year: 57,149,1992
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 5 mg/L
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
12(Suppl 13),103,1988
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - mouse Embryo
DOSE/DURATION : 10 umol/L/24H
REFERENCE :
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
V.1- 1869- Volume(issue)/page/year: 260,368,1976
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - mouse Cells - not otherwise specified
DOSE/DURATION : 10 umol/L
REFERENCE :
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
V.1- 1869- Volume(issue)/page/year: 260,368,1976
TYPE OF TEST : DNA damage
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 5970 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 203,155,1988
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 25 mg/L
REFERENCE :
JEPTDQ Journal of Environmental Pathology and Toxicology. (Park Forest
South, IL) V.1-5(3), 1977-81(?). For publisher information, see JEPOEC.
Volume(issue)/page/year: 1(1),87,1977
TYPE OF TEST : Host-mediated assay
TEST SYSTEM : Rodent - mouse Bacteria - Escherichia coli
DOSE/DURATION : 160 mg/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 272,161,1992
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - hamster Embryo
DOSE/DURATION : 500 ug/L
REFERENCE :
NCIMAV National Cancer Institute, Monograph. (U.S. Government Printing
Office, Supt. of Documents, Washington, DC 20402) No.1- 1959-
Volume(issue)/page/year: 58,243,1981
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 123 mg/L
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
27,67,1996
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Fibroblast
DOSE/DURATION : 8 mg/L/48H
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 48,337,1977
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Cells - not otherwise specified
DOSE/DURATION : 10 umol/L/24H
REFERENCE :
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
V.1- 1869- Volume(issue)/page/year: 260,368,1976
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Cells - not otherwise specified
DOSE/DURATION : 10 umol/L
REFERENCE :
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736)
V.1- 1869- Volume(issue)/page/year: 260,368,1976
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 10 mg/L
REFERENCE :
ATSUDG Archives of Toxicology, Supplement. (Springer-Verlag New York, Inc.,
Service Center, 44 Hartz Way, Secaucus, NJ 07094) No.1- 1978-
Volume(issue)/page/year: 4,41,1980
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 10 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 56,207,1977
TYPE OF TEST : Sister chromatid exchange
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - hamster
DOSE/DURATION : 125 mg/kg
REFERENCE :
BLFSBY Basic Life Sciences. (Plenum Pub. Corp., 223 Spring St., New York,
NY 10003) V.1- 1973- Volume(issue)/page/year: 29B,613,1984
*** REVIEWS ***
IARC Cancer Review:Animal Limited Evidence
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 57,185,1993
IARC Cancer Review:Animal Inadequate Evidence
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 16,73,1978
IARC Cancer Review:Human No Adequate Data
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 16,73,1978
IARC Cancer Review:Human Inadequate Evidence
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 57,185,1993
IARC Cancer Review:Group 3
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 57,185,1993
TOXICOLOGY REVIEW
JACTDZ Journal of the American College of Toxicology. (Mary Ann Liebert,
Inc., 1651 Third Ave., New York, NY 10128) V.1-12, 1982-1993. Discontinued.
Volume(issue)/page/year: 4(3),161,1985
*** OCCUPATIONAL EXPOSURE LIMITS ***
OEL-GERMANY;Skin;Carcinogen JAN 1993
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - X1644
No. of Facilities: 3160 (estimated)
No. of Industries: 1
No. of Occupations: 1
No. of Employees: 29422 (estimated)
No. of Female Employees: 23531 (estimated)
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: SHE-clonal assay; Cell
transform.-C3H/10T1/2
EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test
EPA GENETOX PROGRAM 1988, Positive/dose response: In vitro
cytogenetics-nonhuman
EPA GENETOX PROGRAM 1988, Positive/dose response: In vitro SCE-nonhuman
EPA GENETOX PROGRAM 1988, Positive/limited: Carcinogenicity-mouse/rat
EPA GENETOX PROGRAM 1988, Negative: Rodent dominant lethal
EPA GENETOX PROGRAM 1988, Inconclusive: Mammalian micronucleus; In vitro
UDS-human fibroblast
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA 8(a) PRELIMINARY ASSESSMENT INFORMATION, PROPOSED RULE
FEREAC Federal Register. (U.S. Government Printing Office, Supt. of
Documents, Washington, DC 20402) V.1- 1936- Volume(issue)/page/year:
47,27009,82
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998
NCI Carcinogenesis Bioassay (feed);clear evidence:mouse
NCITR* National Cancer Institute Carcinogenesis Technical Report Series.
(Bethesda, MD) No.0-205. For publisher information, see NTPTR*.
Volume(issue)/page/year: NCI-TR-169,79
NCI Carcinogenesis Bioassay (feed);no evidence:rat
NCITR* National Cancer Institute Carcinogenesis Technical Report Series.
(Bethesda, MD) No.0-205. For publisher information, see NTPTR*.
Volume(issue)/page/year: NCI-TR-169,79
*** END OF RECORD ***