*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : SO5600000
CHEMICAL NAME : 3H-Phenothiazine, 7-(dimethylamino)-3-(methylimino)-,
3-methochloride
CAS REGISTRY NUMBER : 61-73-4
LAST UPDATED : 199710
DATA ITEMS CITED : 36
MOLECULAR FORMULA : C16-H18-N3-S.Cl
MOLECULAR WEIGHT : 319.88
WISWESSER LINE NOTATION : T C666 BS EY INJ EUK1&1 MN1&1 &G &421
COMPOUND DESCRIPTOR : Drug
Mutagen
Reproductive Effector
Human
SYNONYMS/TRADE NAMES :
* Aizen Methylene Blue BH
* Aizen Methylene Blue FZ
* Basic Blue 9
* 3,7-Bis (dimethylamino) phenazathionium chloride
* 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride
* Calcozine Blue ZF
* Chromosmon
* C.I. 52015
* C.I. Basic Blue 9
* D and C Blue No. 1
* Ext D and C Blue No. 1
* External Blue 1
* Hidaco Methylene Blue Salt Free
* Leather Pure Blue HB
* Methylenblau
* Methylene Blue
* Methylene Blue A
* Methylene Blue B
* Methylene Blue 2B
* Methylene Blue BB
* Methylene Blue BBA
* Methylene Blue BB (zinc free)
* Methylene Blue BD
* Methylene Blue 2BF
* Methylene Blue 2BN
* Methylene Blue BP
* Methylene Blue 2BP
* Methylene Blue BPC
* Methylene Blue BX
* Methylene Blue BZ
* Methylene Blue chloride
* Methylene Blue chloride (biological stain)
* Methylene Blue D
* Methylene Blue FZ
* Methylene Blue G
* Methylene Blue GZ
* Methylene Blue HGG
* Methylene Blue IAD
* Methylene Blue I (medicinal)
* Methylene Blue JFA
* Methylene Blue (medicinal)
* Methylene Blue N
* Methylene Blue NF (medicinal)
* Methylene Blue NZ
* Methylene Blue Polychrome
* Methylene Blue SG
* Methylene Blue SP
* Methylene Blue USP (medicinal)
* Methylene Blue USP XII (medicinal)
* Methylene Blue ZF
* Methylene Blue Zinc Free
* Methylene Blue ZX
* Methylenium ceruleum
* Methylthionine
* Methylthionine chloride
* Methylthionium chloride
* Mitsui Methylene Blue
* Modr Methylenova
* Modr Rozpoustedlova 8
* Modr Zasadita 9
* Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride
* Sandocryl Blue BRL
* Schultz No. 1038
* Swiss Blue
* Tetramethylene Blue
* Tetramethylthionine chloride
* Yamamoto Methylene Blue B
* Yamamoto Methylene Blue ZF
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Unreported
SPECIES OBSERVED : Human - infant
DOSE/DURATION : 15 mg/kg
TOXIC EFFECTS :
Lungs, Thorax, or Respiration - cyanosis
Blood - other changes
REFERENCE :
34ZIAG "Toxicology of Drugs and Chemicals," Deichmann, W.B., New York,
Academic Press, Inc., 1969 Volume(issue)/page/year: -,390,1969
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 1180 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
85JCAE "Prehled Prumyslove Toxikologie; Organicke Latky," Marhold, J.,
Prague, Czechoslovakia, Avicenum, 1986 Volume(issue)/page/year: -,1334,1986
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 180 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
AEPPAE Naunyn-Schmiedeberg's Archiv fuer Experimentelle Pathologie und
Pharmakologie. (Berlin, Ger.) V.110-253, 1925-66. For publisher
information, see NSAPCC. Volume(issue)/page/year: 204,288,1947
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 190 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NIIRDN Drugs in Japan (Ethical Drugs). (Yakugyo Jiho Co., Ltd., Tokyo,
Japan) Volume(issue)/page/year: -,1185,1990
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 1250 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
ARZNAD Arzneimittel-Forschung. Drug Research. (Editio Cantor Verlag,
Postfach 1255, W-7960 Aulendorf, Fed. Rep. Ger.) V.1- 1951-
Volume(issue)/page/year: 18,676,1968
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 3500 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
CKFRAY Ceskoslovenska Farmacie. (PNS-Ustredni Expedice a Dovoz Tisku,
Kafkova 19, 160 00 Prague 6, Czechoslovakia) V.1- 1952-
Volume(issue)/page/year: 12,94,1963
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 150 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NTIS** National Technical Information Service. (Springfield, VA 22161)
Formerly U.S. Clearinghouse for Scientific & Technical Information.
Volume(issue)/page/year: AD691-490
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 77 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
CKFRAY Ceskoslovenska Farmacie. (PNS-Ustredni Expedice a Dovoz Tisku,
Kafkova 19, 160 00 Prague 6, Czechoslovakia) V.1- 1952-
Volume(issue)/page/year: 12,94,1963
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Mammal - dog
DOSE/DURATION : 500 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
HBAMAK "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." (Leipzig,
Ger. Dem. Rep.) Volume(issue)/page/year: 4,1366,1935
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Mammal - dog
DOSE/DURATION : 50 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
HBAMAK "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." (Leipzig,
Ger. Dem. Rep.) Volume(issue)/page/year: 4,1366,1935
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Primate - monkey
DOSE/DURATION : 10 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
HBAMAK "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." (Leipzig,
Ger. Dem. Rep.) Volume(issue)/page/year: 4,1366,1935
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Mammal - cat
DOSE/DURATION : 41 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
AIMEAS Annals of Internal Medicine. (American College of Physicians, 4200
Pine St., Philadelphia, PA 19104) V.1- 1927- Volume(issue)/page/year:
7,738,1933
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rabbit
DOSE/DURATION : 1 gm/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
HBAMAK "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." (Leipzig,
Ger. Dem. Rep.) Volume(issue)/page/year: 4,1366,1935
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - guinea pig
DOSE/DURATION : 300 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
HBAMAK "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." (Leipzig,
Ger. Dem. Rep.) Volume(issue)/page/year: 4,1366,1935
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Mammal - domestic
DOSE/DURATION : 42300 ug/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
JVPTD9 Journal of Veterinary Pharmacology and Therapeutics. (Blackwell
Scientific Pub. Ltd., POB 88, Oxford, UK) V.1- 1978-
Volume(issue)/page/year: 7,225,1984
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 2500 mg/kg
SEX/DURATION : female 1-22 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - post-implantation mortality (e.g. dead and/or
resorbed implants per total number of implants)
REFERENCE :
AJANA2 American Journal of Anatomy. (Alan R. Liss, Inc., 41 E. 11th St.,
New York, NY 10003) V.1-192, 1901-91. Volume(issue)/page/year: 110,29,1962
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 20 ug/plate
REFERENCE :
ABCHA6 Agricultural and Biological Chemistry. (Maruzen Co. Ltd., POB 5050,
Tokyo International, Tokyo 100-31, Japan) V.25- 1961-
Volume(issue)/page/year: 45,327,1981
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 10 pph
REFERENCE :
AGACBH Agents and Actions, A Swiss Journal of Pharmacology. (Birkhaeuser
Verlag, POB 133, CH-4010 Basel, Switzerland) V.1- 1969/70-
Volume(issue)/page/year: 4,286,1974
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 2 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 137,1,1984
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 160 ug/well
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 3,429,1981
TYPE OF TEST : DNA adduct
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 70 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 89,95,1981
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Bacillus subtilis
DOSE/DURATION : 2 mg/disc
REFERENCE :
TRENAF Kenkyu Nenpo--Tokyo-toritsu Eisei Kenkyusho. Annual Report of Tokyo
Metropolitan Research Laboratory of Public Health. (Tokyo-toritsu Eisei
Kenkyusho, 24-1, 3-chome, Hyakunin-cho, Shin-juku-ku, Tokyo 160, Japan) V.1-
1949/50- Volume(issue)/page/year: 27,153,1976
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Microorganism - not otherwise specified
DOSE/DURATION : 20 ppm
REFERENCE :
POASAD Proceedings of the Oklahoma Academy of Science. (Oklahoma Academy of
Science, c/o Executive Secretary-Treasurer, Southwestern Oklahoma State
Univ., Weatherford, OK 73096) V.1- 1910/1920- Volume(issue)/page/year:
34,114,1953
TYPE OF TEST : Gene conversion and mitotic recombination
TEST SYSTEM : Yeast - Saccharomyces cerevisiae
DOSE/DURATION : 10 pph
REFERENCE :
AGACBH Agents and Actions, A Swiss Journal of Pharmacology. (Birkhaeuser
Verlag, POB 133, CH-4010 Basel, Switzerland) V.1- 1969/70-
Volume(issue)/page/year: 4,286,1974
TYPE OF TEST : DNA adduct
TEST SYSTEM : Rodent - mouse Ascites tumor
DOSE/DURATION : 70 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 89,95,1981
TYPE OF TEST : DNA adduct
TEST SYSTEM : Mammal - species unspecified Lymphocyte
DOSE/DURATION : 1830 nmol/L
REFERENCE :
BIPMAA Biopolymers. (John Wiley & Sons, Inc., 605 Third Ave., New York, NY
10158) V.1- 1963- Volume(issue)/page/year: 12,1099,1973
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOHS - National Occupational Hazard Survey (1974)
NOHS Hazard Code - M1469
No. of Facilities: 4471 (estimated)
No. of Industries: 35
No. of Occupations: 34
No. of Employees: 25916 (estimated)
NOHS - National Occupational Hazard Survey (1974)
NOHS Hazard Code - T1406
No. of Facilities: 150 (estimated)
No. of Industries: 2
No. of Occupations: 2
No. of Employees: 664 (estimated)
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - M1469
No. of Facilities: 5956 (estimated)
No. of Industries: 29
No. of Occupations: 51
No. of Employees: 74208 (estimated)
No. of Female Employees: 43305 (estimated)
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive/dose response: In vitro SCE-nonhuman
EPA GENETOX PROGRAM 1988, Negative: B subtilis rec assay; S cerevisiae gene
conversion
EPA GENETOX PROGRAM 1988, Inconclusive: B subtilis rec assay; Histidine
reversion-Ames test
EPA GENETOX PROGRAM 1988, Inconclusive: D melanogaster Sex-linked lethal
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(e) Risk Notification, 8EHQ-0892-8826
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998
*** END OF RECORD ***