*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : PA2975000
CHEMICAL NAME : Methane, azoxy-
CAS REGISTRY NUMBER : 25843-45-2
LAST UPDATED : 199503
DATA ITEMS CITED : 37
MOLECULAR FORMULA : C2-H6-N2-O
MOLECULAR WEIGHT : 74.10
WISWESSER LINE NOTATION : ON1&UN1
COMPOUND DESCRIPTOR : Tumorigen
Mutagen
Reproductive Effector
SYNONYMS/TRADE NAMES :
* AOM
* Azoxymethane
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 27 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
XENOBH Xenobiotica. (Taylor & Francis Ltd., 4 John St., London WC1N 2ET,
UK) V.1- 1971- Volume(issue)/page/year: 3,271,1973
** TUMORIGENIC DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 20 mg/kg/(22D
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Reproductive - Tumorigenic effects - transplacental tumorigenesis
Peripheral Nerve and Sensation - peripheral nerve tumors
REFERENCE :
XENOBH Xenobiotica. (Taylor & Francis Ltd., 4 John St., London WC1N 2ET,
UK) V.1- 1971- Volume(issue)/page/year: 3,271,1973
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 16 mg/kg
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Gastrointestinal - colon tumors
Kidney, Ureter, Bladder - Kidney tumors
REFERENCE :
23HZAR "Carcinoma of the Colon and Antecedent Epithelium," Burdette, W.J.,
ed., Springfield, IL C.C. Thomas, 1970 Volume(issue)/page/year: -,267,1970
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 3200 ug/kg
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Gastrointestinal - tumors
Gastrointestinal - colon tumors
REFERENCE :
VTPHAK Veterinary Pathology. (Waverly Press, Inc., POB 64025, Baltimore, MD
21264) V.8- 1971- Volume(issue)/page/year: 12,165,1975
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 20 mg/kg
female 22 day(s) after conception
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Reproductive - Tumorigenic effects - transplacental tumorigenesis
Kidney, Ureter, Bladder - Kidney tumors
REFERENCE :
IARCCD IARC Scientific Publications. (Geneva, Switzerland) No.1-26,
1971-78. For publisher information, see IAPUDO. Volume(issue)/page/year:
4,45,1973
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intramuscular
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 22 mg/kg/11W-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Gastrointestinal - tumors
REFERENCE :
JJIND8 JNCI, Journal of the National Cancer Institute. (Washington, DC)
V.61-79, 1978-87. For publisher information, see JNCIEQ.
Volume(issue)/page/year: 73,1297,1984
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Rectal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 200 mg/kg/20W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Gastrointestinal - tumors
Gastrointestinal - colon tumors
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
35,287,1975
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 96 mg/kg/13W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Gastrointestinal - tumors
Gastrointestinal - colon tumors
REFERENCE :
JAVMA4 Journal of the American Veterinary Medical Association. (American
Veterinary Medical Assoc., 930 N. Meacham Rd., Schaumburg, IL 60196) V.48-
1915- Volume(issue)/page/year: 164,729,1974
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Rectal
SPECIES OBSERVED : Rodent - guinea pig
DOSE/DURATION : 104 mg/kg/33W-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Liver - tumors
REFERENCE :
JNCIAM Journal of the National Cancer Institute. (Washington, DC) V.1-60,
1940-78. For publisher information, see JJIND8. Volume(issue)/page/year:
56,653,1976
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - hamster
DOSE/DURATION : 60 mg/kg/20W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Liver - tumors
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
47,3968,1987
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 312 mg/kg/39W-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Gastrointestinal - tumors
REFERENCE :
DICRAG Diseases of the Colon and Rectum. (Lippincott/Harper, Journal
Fulfillment Dept., 2350 Virginia Ave., Hagerstown, MD 21740) V.1- 1958-
Volume(issue)/page/year: 16,438,1973
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 80 mg/kg/10W-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Gastrointestinal - colon tumors
Gastrointestinal - tumors
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
38,4427,1978
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 20 mg/kg
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Gastrointestinal - colon tumors
REFERENCE :
JJIND8 JNCI, Journal of the National Cancer Institute. (Washington, DC)
V.61-79, 1978-87. For publisher information, see JNCIEQ.
Volume(issue)/page/year: 72,745,1984
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 64 mg/kg/8W-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Gastrointestinal - tumors
Gastrointestinal - colon tumors
REFERENCE :
JJIND8 JNCI, Journal of the National Cancer Institute. (Washington, DC)
V.61-79, 1978-87. For publisher information, see JNCIEQ.
Volume(issue)/page/year: 73,275,1984
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 12 mg/kg
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Gastrointestinal - colon tumors
REFERENCE :
FEPRA7 Federation Proceedings, Federation of American Societies for
Experimental Biology. (Bethesda, MD) V.1-46, 1942-87.
Volume(issue)/page/year: 31,1482,1972
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 45 mg/kg/3W-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Gastrointestinal - colon tumors
Liver - tumors
REFERENCE :
JJIND8 JNCI, Journal of the National Cancer Institute. (Washington, DC)
V.61-79, 1978-87. For publisher information, see JNCIEQ.
Volume(issue)/page/year: 75,791,1985
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 208 mg/kg/26W-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Gastrointestinal - tumors
REFERENCE :
JNCIAM Journal of the National Cancer Institute. (Washington, DC) V.1-60,
1940-78. For publisher information, see JJIND8. Volume(issue)/page/year:
54,439,1975
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 192 mg/kg/24W-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Lungs, Thorax, or Respiration - tumors
Gastrointestinal - tumors
REFERENCE :
JJIND8 JNCI, Journal of the National Cancer Institute. (Washington, DC)
V.61-79, 1978-87. For publisher information, see JNCIEQ.
Volume(issue)/page/year: 62,1097,1979
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 80 mg/kg/10W-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Gastrointestinal - tumors
Gastrointestinal - colon tumors
REFERENCE :
JJIND8 JNCI, Journal of the National Cancer Institute. (Washington, DC)
V.61-79, 1978-87. For publisher information, see JNCIEQ.
Volume(issue)/page/year: 66,553,1981
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 37 mg/kg/10W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Gastrointestinal - tumors
Gastrointestinal - colon tumors
REFERENCE :
JAVMA4 Journal of the American Veterinary Medical Association. (American
Veterinary Medical Assoc., 930 N. Meacham Rd., Schaumburg, IL 60196) V.48-
1915- Volume(issue)/page/year: 164,729,1974
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE : 30 mg/kg
SEX/DURATION : female 14 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetal death
REFERENCE :
IARCCD IARC Scientific Publications. (Geneva, Switzerland) No.1-26,
1971-78. For publisher information, see IAPUDO. Volume(issue)/page/year:
4,45,1973
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intravenous
SPECIES OBSERVED : Rodent - rat
DOSE : 30 mg/kg
SEX/DURATION : female 15 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetal death
REFERENCE :
IARCCD IARC Scientific Publications. (Geneva, Switzerland) No.1-26,
1971-78. For publisher information, see IAPUDO. Volume(issue)/page/year:
4,45,1973
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 300 ng/plate
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 6(Suppl
2),1,1984
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 10 ug/plate
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 6(Suppl
2),1,1984
TYPE OF TEST : Sex chromosome loss and nondisjunction
ROUTE OF EXPOSURE : Unreported
TEST SYSTEM : Insect - Drosophila melanogaster
DOSE/DURATION : 1 mmol/L/3D (Continuous)
REFERENCE :
DRISAA Drosophila Information Service. (Cold Spring Harbor Laboratory, POB
100, Cold Spring Harbor, NY 11724) No.1- 1934- Volume(issue)/page/year:
50,138,1973
TYPE OF TEST : Sex chromosome loss and nondisjunction
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Insect - Drosophila melanogaster
DOSE/DURATION : 1300 umol/L/3D (Intermittent)
REFERENCE :
35WYAM "In Vitro Metabolic Activation in Mutagenic Testing, Proceedings of
the Symposium, Research Triangle Park, NC, 1976," De Serres, F.J., et al.,
eds., New York, Elsevier Science Pub. Co., Inc., 1976
Volume(issue)/page/year: -,63,1976
TYPE OF TEST : Morphological transformation
ROUTE OF EXPOSURE : Subcutaneous
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 30 mg/kg/14D (Intermittent)
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 15,1049,1994
TYPE OF TEST : DNA damage
ROUTE OF EXPOSURE : Subcutaneous
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 30 mg/kg
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
38,1589,1978
TYPE OF TEST : DNA damage
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 10 mg/kg
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 4,667,1982
TYPE OF TEST : Unscheduled DNA synthesis
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 10 mg/kg
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 4,553,1982
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 1 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 221,263,1989
TYPE OF TEST : Body fluid assay
TEST SYSTEM : Rodent - rat Bacteria - Salmonella typhimurium
DOSE/DURATION : 12800 ug/kg
REFERENCE :
PNASA6 Proceedings of the National Academy of Sciences of the United States
of America. (National Academy of Sciences, Printing & Pub. Office, 2101
Constitution Ave., Washington, DC 20418) V.1- 1915-
Volume(issue)/page/year: 72,4607,1975
TYPE OF TEST : Host-mediated assay
TEST SYSTEM : Rodent - mouse Bacteria - Salmonella typhimurium
DOSE/DURATION : 250 umol/kg
REFERENCE :
JJIND8 JNCI, Journal of the National Cancer Institute. (Washington, DC)
V.61-79, 1978-87. For publisher information, see JNCIEQ.
Volume(issue)/page/year: 63,977,1979
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: Carcinogenicity-mouse/rat; Host-mediated
assay
EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test
EPA GENETOX PROGRAM 1988, Positive: D melanogaster Sex-linked lethal
EPA GENETOX PROGRAM 1988, Negative: Cell transform.-BALB/c-3T3
*** END OF RECORD ***