*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : NJ1050000
CHEMICAL NAME : 2-Imidazolidinone, 1-(5-nitro-2-thiazolyl)-
CAS REGISTRY NUMBER : 61-57-4
BEILSTEIN REFERENCE NO. : 0665338
LAST UPDATED : 199612
DATA ITEMS CITED : 43
MOLECULAR FORMULA : C6-H6-N4-O3-S
MOLECULAR WEIGHT : 214.22
WISWESSER LINE NOTATION : T5N CSJ DNW B- CT5MVNTJ
COMPOUND DESCRIPTOR : Tumorigen
Drug
Mutagen
Reproductive Effector
SYNONYMS/TRADE NAMES :
* Ambilhar
* BA 32644
* BA 32644 Ciba
* Ciba 32644
* Ciba 32644-Ba
* Niridazole
* Nitridazole
* Nitrothiamidazol
* Nitrothiamidazole
* Nitrothiazole
* 1-(5-Nitro-2-thiazolyl)imidazolidin-2-one
* 1-(5-Nitro-2-thiazolyl)-2-imidazolidinone
* 1-(5-Nitro-2-thiazolyl)-2-imidazolinone
* 1-(5-Nitro-2-thiazolyl)-2-oxotetrahydroimidazol
* 1-(5-Nitro-2-thiazolyl)-2-oxotetrahydroimidazole
* NTOI
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 900 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
FAZMAE Fortschritte der Arzneimittelforschung. Progress in Drug Research.
(Birkhauser Boston, Inc., c/o Springer-Verlag New York, 44 Hartz Way,
Secaucus, NJ 07094) V.1- 1959- Volume(issue)/page/year: 17,108,1973
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 2500 mg/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold
Lungs, Thorax, or Respiration - respiratory depression
REFERENCE :
YHHPAL Yaoxue Xuebao. Acta Pharmaceutica Sinica. Pharmaceutical Journal.
(China International Book Trading Corp., POB 2820, Beijing, Peop. Rep.
China) V.1- 1953- Suspended 1966-78. Volume(issue)/page/year:
15,456,1980
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 220 mg/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold
Behavioral - ataxia
Kidney, Ureter, Bladder - hematuria
REFERENCE :
JPETAB Journal of Pharmacology and Experimental Therapeutics. (Williams &
Wilkins Co., 428 E. Preston St., Baltimore, MD 21202) V.1- 1909/10-
Volume(issue)/page/year: 228,662,1984
** TUMORIGENIC DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 8 gm/kg/90W-I
TOXIC EFFECTS :
Tumorigenic - neoplastic by RTECS criteria
Gastrointestinal - tumors
Kidney, Ureter, Bladder - Kidney tumors
REFERENCE :
CALEDQ Cancer Letters (Shannon, Ireland). (Elsevier Scientific Pub. Ireland
Ltd., POB 85, Limerick, Ireland) V.1- 1975- Volume(issue)/page/year:
4,305,1978
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 15 gm/kg/49W-C
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Lungs, Thorax, or Respiration - tumors
Gastrointestinal - tumors
REFERENCE :
CALEDQ Cancer Letters (Shannon, Ireland). (Elsevier Scientific Pub. Ireland
Ltd., POB 85, Limerick, Ireland) V.1- 1975- Volume(issue)/page/year:
1,69,1975
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - hamster
DOSE/DURATION : 21 gm/kg/63W-C
TOXIC EFFECTS :
Tumorigenic - neoplastic by RTECS criteria
Gastrointestinal - tumors
Kidney, Ureter, Bladder - tumors
REFERENCE :
JNCIAM Journal of the National Cancer Institute. (Washington, DC) V.1-60,
1940-78. For publisher information, see JJIND8. Volume(issue)/page/year:
59,1625,1977
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 16 gm/kg/75W-C
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Lungs, Thorax, or Respiration - tumors
Gastrointestinal - tumors
REFERENCE :
JNCIAM Journal of the National Cancer Institute. (Washington, DC) V.1-60,
1940-78. For publisher information, see JJIND8. Volume(issue)/page/year:
59,1625,1977
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 4 gm/kg/19W-C
TOXIC EFFECTS :
Tumorigenic - neoplastic by RTECS criteria
Lungs, Thorax, or Respiration - tumors
Gastrointestinal - tumors
REFERENCE :
CALEDQ Cancer Letters (Shannon, Ireland). (Elsevier Scientific Pub. Ireland
Ltd., POB 85, Limerick, Ireland) V.1- 1975- Volume(issue)/page/year:
1,69,1975
** REPRODUCTIVE DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 350 mg/kg
SEX/DURATION : male 5 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Fertility - pre-implantation mortality (e.g. reduction in
number of implants per female; total number of implants per corpora lutea)
REFERENCE :
JRPFA4 Journal of Reproduction and Fertility. (Biochemical Soc. Book Depot,
POB 32, Commerce Way, Colchester, Essex CO2 8HP, UK) V.1- 1960-
Volume(issue)/page/year: 46,217,1976
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 500 mg/kg
SEX/DURATION : male 5 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Fertility - male fertility index (e.g. # males impregnating
females per # males exposed to fertile nonpregnant females)
REFERENCE :
JRPFA4 Journal of Reproduction and Fertility. (Biochemical Soc. Book Depot,
POB 32, Commerce Way, Colchester, Essex CO2 8HP, UK) V.1- 1960-
Volume(issue)/page/year: 46,217,1976
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE : 500 mg/kg
SEX/DURATION : male 5 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Paternal Effects - spermatogenesis (incl. genetic material,
sperm morphology, motility, and count)
REFERENCE :
ACTRAQ Acta Tropica. (Schwabe & Co., Steintorstr. 13, CH-4010 Basel,
Switzerland) V.1- 1944- Volume(issue)/page/year: 22,155,1965
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE : 450 mg/kg
SEX/DURATION : male 18 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Paternal Effects - testes, epididymis, sperm duct
Reproductive - Paternal Effects - prostate, seminal vesicle, Cowper's gland,
accessory glands
REFERENCE :
TOLED5 Toxicology Letters. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1977- Volume(issue)/page/year: 14,163,1982
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE : 625 mg/kg
SEX/DURATION : male 5 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Fertility - pre-implantation mortality (e.g. reduction in
number of implants per female; total number of implants per corpora lutea)
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
23,288,1972
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE : 175 mg/kg
SEX/DURATION : male 5 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Fertility - male fertility index (e.g. # males impregnating
females per # males exposed to fertile nonpregnant females)
Reproductive - Fertility - pre-implantation mortality (e.g. reduction in
number of implants per female; total number of implants per corpora lutea)
REFERENCE :
JRPFA4 Journal of Reproduction and Fertility. (Biochemical Soc. Book Depot,
POB 32, Commerce Way, Colchester, Essex CO2 8HP, UK) V.1- 1960-
Volume(issue)/page/year: 46,217,1976
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE : 700 mg/kg
SEX/DURATION : male 1 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Fertility - male fertility index (e.g. # males impregnating
females per # males exposed to fertile nonpregnant females)
REFERENCE :
EXPEAM Experientia. (Birkhaeuser Verlag, POB 133, CH-4010 Basel,
Switzerland) V.1- 1945- Volume(issue)/page/year: 29,1253,1973
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE : 600 mg/kg
SEX/DURATION : male 1 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Fertility - pre-implantation mortality (e.g. reduction in
number of implants per female; total number of implants per corpora lutea)
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
23,288,1972
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intratesticular
SPECIES OBSERVED : Mammal - dog
DOSE : 18 mg/kg
SEX/DURATION : male 1 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Paternal Effects - testes, epididymis, sperm duct
REFERENCE :
IJEBA6 Indian Journal of Experimental Biology. (Publications & Information
Directorate, CSIR, Hillside Rd., New Delhi 110 012, India) V.1- 1963-
Volume(issue)/page/year: 17,937,1979
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - guinea pig
DOSE : 250 mg/kg
SEX/DURATION : male 5 day(s) pre-mating
TOXIC EFFECTS :
Reproductive - Paternal Effects - spermatogenesis (incl. genetic material,
sperm morphology, motility, and count)
Reproductive - Paternal Effects - testes, epididymis, sperm duct
REFERENCE :
JRPFA4 Journal of Reproduction and Fertility. (Biochemical Soc. Book Depot,
POB 32, Commerce Way, Colchester, Essex CO2 8HP, UK) V.1- 1960-
Volume(issue)/page/year: 48,439,1976
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 12500 pg/plate
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 130,79,1984
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 33 ng/plate
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 7,429,1985
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 100 ng/plate
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 142,163,1985
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 12500 ug/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 280,93,1992
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 600 ng/disc
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 97,1,1982
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Bacillus subtilis
DOSE/DURATION : 60 ng/disc
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 97,1,1982
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Klebsiella pneumoniae
DOSE/DURATION : 500 nmol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 118,153,1983
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Mold - Neurospora crassa
DOSE/DURATION : 200 mg/L
REFERENCE :
JEPTDQ Journal of Environmental Pathology and Toxicology. (Park Forest
South, IL) V.1-5(3), 1977-81(?). For publisher information, see JEPOEC.
Volume(issue)/page/year: 2(4),1109,1979
TYPE OF TEST : Gene conversion and mitotic recombination
TEST SYSTEM : Yeast - Saccharomyces cerevisiae
DOSE/DURATION : 100 mg/L/22H
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 55,43,1978
TYPE OF TEST : Body fluid assay
TEST SYSTEM : Human Bacteria - Salmonella typhimurium
DOSE/DURATION : 25 mg/kg
REFERENCE :
CMMUAO Chemical Mutagens. Principles and Methods for Their Detection.
(Plenum Pub. Corp., 233 Spring St., New York, NY 10013) V.1- 1971-
Volume(issue)/page/year: 4,171,1976
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 10 mg/L
REFERENCE :
CBTOE2 Cell Biology and Toxicology. (Princeton Scientific Pub., Inc., 301
N. Harrison St., CN 5279, Princeton, NJ 08540) V.1- 1984-
Volume(issue)/page/year: 2,379,1986
TYPE OF TEST : DNA damage
TEST SYSTEM : Rodent - mouse Fibroblast
DOSE/DURATION : 300 umol/L
REFERENCE :
BJCSB5 British Journal of Cancer, Supplement. (Macmillan Press Ltd.,
Houndmills, Basingstoke, Hants. RG21 2ZS, UK) No.1- 1973-
Volume(issue)/page/year: 3,124,1978
TYPE OF TEST : Body fluid assay
TEST SYSTEM : Rodent - mouse Bacteria - Salmonella typhimurium
DOSE/DURATION : 200 mg/kg
REFERENCE :
CMMUAO Chemical Mutagens. Principles and Methods for Their Detection.
(Plenum Pub. Corp., 233 Spring St., New York, NY 10013) V.1- 1971-
Volume(issue)/page/year: 4,171,1976
TYPE OF TEST : Body fluid assay
TEST SYSTEM : Rodent - mouse Insect - Drosophila melanogaster
DOSE/DURATION : 200 mg/kg
REFERENCE :
CMMUAO Chemical Mutagens. Principles and Methods for Their Detection.
(Plenum Pub. Corp., 233 Spring St., New York, NY 10013) V.1- 1971-
Volume(issue)/page/year: 4,171,1976
TYPE OF TEST : Host-mediated assay
TEST SYSTEM : Rodent - mouse Bacteria - Salmonella typhimurium
DOSE/DURATION : 1 mg/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 55,43,1978
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Rodent - mouse Bacteria - Escherichia coli
DOSE/DURATION : 125 mg/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 280,93,1992
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 10 mg/L
REFERENCE :
CBTOE2 Cell Biology and Toxicology. (Princeton Scientific Pub., Inc., 301
N. Harrison St., CN 5279, Princeton, NJ 08540) V.1- 1984-
Volume(issue)/page/year: 2,379,1986
TYPE OF TEST : Sperm Morphology
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - guinea pig
DOSE/DURATION : 250 mg/kg/5D (Continuous)
REFERENCE :
JRPFA4 Journal of Reproduction and Fertility. (Biochemical Soc. Book Depot,
POB 32, Commerce Way, Colchester, Essex CO2 8HP, UK) V.1- 1960-
Volume(issue)/page/year: 48,439,1976
*** REVIEWS ***
IARC Cancer Review:Animal Sufficient Evidence
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 13,123,1977
IARC Cancer Review:Human No Adequate Data
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 13,123,1977
IARC Cancer Review:Group 2B
IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49
Sheridan Ave., Albany, NY 12210) No.1- 1979- Volume(issue)/page/year:
7,56,1987
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: Carcinogenicity-mouse/rat; N
crassa-forward mutation
EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test; S
cerevisiae-homozygosis
EPA GENETOX PROGRAM 1988, Positive/dose response: TRP reversion
EPA GENETOX PROGRAM 1988, Negative: Sperm morphology-human
*** END OF RECORD ***