*** CHEMICAL IDENTIFICATION ***

RTECS NUMBER            : AR7601000
CHEMICAL NAME           : Acridine, 3,6-bis(dimethylamino)-, monohydrochloride
CAS REGISTRY NUMBER     : 65-61-2
LAST UPDATED            : 199701
DATA ITEMS CITED        : 20
MOLECULAR FORMULA       : C17-H19-N3.Cl-H
MOLECULAR WEIGHT        : 301.85
WISWESSER LINE NOTATION : T C666 BNJ EN1&1 MN1&1 &GH
COMPOUND DESCRIPTOR     : Mutagen
SYNONYMS/TRADE NAMES :
   * 3,6-Acridinediamine, N,N,N',N'-tetramethyl-, monohydrochloride
   * Acridine Orange
   * Acridine Orange N
   * Acridine Orange NO
   * Acridine Orange NS
   * Acridine Orange R
   * Basic Orange 14
   * Basic Orange 3RN
   * C.I. 46005
   * C.I. Basic Orange 14
   * Rhoduline Orange NO
 
                       *** HEALTH HAZARD DATA ***
 
                            ** MUTATION DATA **
 
TYPE OF TEST            : Mutation test systems - not otherwise specified
TEST SYSTEM             : Bacteria - Escherichia coli
DOSE/DURATION           : 10 umol/L
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 203,81,1988
 
TYPE OF TEST            : Phage inhibition capacity
TEST SYSTEM             : Bacteria - Escherichia coli
DOSE/DURATION           : 9400 ng/well
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 260,349,1991
 
TYPE OF TEST            : Sex chromosome loss and nondisjunction
ROUTE OF EXPOSURE       : Oral
TEST SYSTEM             : Insect - Drosophila melanogaster
DOSE/DURATION           : 5000 ppm
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 121,199,1983
 
TYPE OF TEST            : Micronucleus test
ROUTE OF EXPOSURE       : Multiple routes
TEST SYSTEM             : Non-mammalian species
DOSE/DURATION           : 700 nmol/L
REFERENCE :
   MUTAEX Mutagenesis. (Oxford Univ. Press, Pinkhill House, Southfield Road,
   Eynsham, Oxford OX8 1JJ, UK) V.1- 1986-  Volume(issue)/page/year: 4,17,1989
 
TYPE OF TEST            : DNA adduct
TEST SYSTEM             : Fish - salmon Sperm
DOSE/DURATION           : 40 nmol/L
REFERENCE :
   BBRCA9 Biochemical and Biophysical Research Communications.  (Academic
   Press, Inc., 1 E. First St., Duluth, MN 55802)  V.1-    1959-
   Volume(issue)/page/year: 40,1239,1970
 
TYPE OF TEST            : DNA damage
TEST SYSTEM             : Rodent - mouse Ascites tumor
DOSE/DURATION           : 20 umol/L
REFERENCE :
   BBACAQ Biochimica et Biophysica Acta.  (Elsevier Science Pub. B.V., POB 211,
   1000 AE Amsterdam, Netherlands)  V.1-    1947- Volume(issue)/page/year:
   374,96,1974
 
TYPE OF TEST            : Morphological transformation
TEST SYSTEM             : Rodent - hamster Embryo
DOSE/DURATION           : 2500 ug/L
REFERENCE :
   AETODY Advances in Modern Environmental Toxicology.  (Senate Press, Inc.,
   P.O. Box 252, Princeton Junction, NJ 08550)  V.1-    1980-
   Volume(issue)/page/year: 1,241,1980
 
TYPE OF TEST            : DNA adduct
TEST SYSTEM             : Mammal - species unspecified Lymphocyte
DOSE/DURATION           : 10 pph
REFERENCE :
   BIPMAA Biopolymers.  (John Wiley & Sons, Inc., 605 Third Ave., New York, NY
   10158)  V.1-    1963-  Volume(issue)/page/year: 11,2537,1972

         *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***

NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
   NOES - National Occupational Exposure Survey (1983)
     NOES Hazard Code - X3803
       No. of Facilities: 29 (estimated)
       No. of Industries: 1
       No. of Occupations: 3
       No. of Employees: 625 (estimated)
       No. of Female Employees: 403 (estimated)
   
   NOES - National Occupational Exposure Survey (1983)
     NOES Hazard Code - X8883
       No. of Facilities: 10 (estimated)
       No. of Industries: 1
       No. of Occupations: 1
       No. of Employees: 114 (estimated)
       No. of Female Employees: 31 (estimated)

                          *** STATUS IN U.S. ***
 
   EPA GENETOX PROGRAM 1988, Positive: Cell transform.-SA7/SHE; Host-mediated
   assay
   
   EPA GENETOX PROGRAM 1988, Positive: Mouse spot test; E coli polA without S9
   
   EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test
   
   EPA GENETOX PROGRAM 1988, Positive: S cerevisiae gene conversion; S
   cerevisiae-homozygosis
   
   EPA GENETOX PROGRAM 1988, Positive/dose response: In vitro SCE-nonhuman
   
   EPA GENETOX PROGRAM 1988, Inconclusive: Carcinogenicity-mouse/rat;
   SHE-clonal assay
   
   EPA GENETOX PROGRAM 1988, Inconclusive: B subtilis rec assay; D melanogaster
   Sex-linked lethal
   
   EPA GENETOX PROGRAM 1988, Inconclusive: S cerevisiae-forward mutation
   
   EPA GENETOX PROGRAM 1988, Positive: CHO gene mutation
   
   EPA TSCA Section 8(b) CHEMICAL INVENTORY

 
                            *** END OF RECORD ***