*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : DD1225000
CHEMICAL NAME : Benzidine, 3,3'-dimethyl-
CAS REGISTRY NUMBER : 119-93-7
BEILSTEIN REFERENCE NO. : 2210640
REFERENCE : 4-13-00-00419 (Beilstein Handbook Reference)
LAST UPDATED : 199712
DATA ITEMS CITED : 49
MOLECULAR FORMULA : C14-H16-N2
MOLECULAR WEIGHT : 212.32
WISWESSER LINE NOTATION : ZR B1 DR DZ C1
COMPOUND DESCRIPTOR : Tumorigen
Mutagen
SYNONYMS/TRADE NAMES :
* Bianisidine
* (1,1'-Biphenyl)-4,4'-diamine-3,3'-dimethyl-
* 4,4'-Bi-o-toluidine
* C.I. 37230
* C.I. Azoic Diazo Component 113
* 4,4'-Diamino-3,3'-dimethylbiphenyl
* 4,4'-Diamino-3,3'-dimethyldiphenyl
* Diaminoditolyl
* 3,3'-Dimethylbenzidin
* 3,3'-Dimethylbenzidine
* 3,3'-Dimethyl-4,4'-biphenyldiamine
* 3,3'-Dimethylbiphenyl-4,4'-diamine
* 3,3'-Dimethyl-4,4'-diphenyldiamine
* 3,3'-Dimethyldiphenyl-4,4'-diamine
* 4,4'-Di-o-toluidine
* Fast Dark Blue Base R
* RCRA waste number U095
* o-Tolidin
* 2-Tolidin
* 2-Tolidina
* Tolidine
* o-Tolidine
* o,o'-Tolidine
* 2-Tolidine
* 3,3'-Tolidine
* o-Tolidine
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 125 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
CBCCT* "Summary Tables of Biological Tests," National Research Council
Chemical-Biological Coordination Center. (National Academy of Science
Library, 2101 Constitution Ave., NW, Washington, DC 20418)
Volume(issue)/page/year: 6,64,1954
TYPE OF TEST : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Mammal - dog
DOSE/DURATION : 600 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
AEXPBL Archiv fuer Experimentelle Pathologie und Pharmakologie. (Leipzig,
Ger. Dem. Rep.) V.1-109, 1873-1925. For publisher information, see NSAPCC.
Volume(issue)/page/year: 58,167,1907
** TUMORIGENIC DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 4500 mg/kg/27D-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Skin and Appendages - tumors
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
28,924,1968
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 1650 mg/kg/33W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Skin and Appendages - tumors
REFERENCE :
VOONAW Voprosy Onkologii. Problems of Oncology. For English translation,
see PONCAU. (V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1-10,
1928-37; V.1- 1955- Volume(issue)/page/year: 20(2),53,1974
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Implant
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 5040 mg/kg/1Y-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Liver - tumors
Skin and Appendages - tumors
REFERENCE :
JNCIAM Journal of the National Cancer Institute. (Washington, DC) V.1-60,
1940-78. For publisher information, see JJIND8. Volume(issue)/page/year:
45,283,1970
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 9 gm/kg/51W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Sense Organs and Special Senses (Ear) - effect, not otherwise specified
REFERENCE :
CANCAR Cancer (Philadelphia). (Lippincott/Harper, Journal Fulfillment
Dept., 2350 Virginia Ave., Hagerstown, MD 21740) V.1- 1948-
Volume(issue)/page/year: 3,789,1950
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 5040 mg/kg/56W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Sense Organs and Special Senses (Ear) - effect, not otherwise specified
Endocrine - tumors
REFERENCE :
GTPZAB Gigiena Truda i Professional'nye Zabolevaniya. Labor Hygiene and
Occupational Diseases. (V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR)
V.1-36, 1957-1992. For publisher information, see MTPEEI
Volume(issue)/page/year: 9(7),18,1965
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 3240 mg/kg/34W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Endocrine - tumors
Skin and Appendages - tumors
REFERENCE :
JNCIAM Journal of the National Cancer Institute. (Washington, DC) V.1-60,
1940-78. For publisher information, see JJIND8. Volume(issue)/page/year:
45,283,1970
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 100 nmol/plate
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 136,33,1984
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 4 ug/plate
REFERENCE :
BJCAAI British Journal of Cancer. (Macmillan Press Ltd., Houndmills,
Basingstoke, Hants. RG21 2XS, UK) V.1- 1947- Volume(issue)/page/year:
37,873,1978
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Human HeLa cell
DOSE/DURATION : 1 umol/L
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
38,2621,1978
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - rat Embryo
DOSE/DURATION : 5 mg/L
REFERENCE :
JNCIAM Journal of the National Cancer Institute. (Washington, DC) V.1-60,
1940-78. For publisher information, see JJIND8. Volume(issue)/page/year:
51,799,1973
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 1 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 136,255,1984
TYPE OF TEST : Body fluid assay
TEST SYSTEM : Rodent - rat Bacteria - Salmonella typhimurium
DOSE/DURATION : 10 ug/plate
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 79,173,1980
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 105 mg/L
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
12(Suppl 13),37,1988
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - mouse Embryo
DOSE/DURATION : 1 mg/L
REFERENCE :
JNCIAM Journal of the National Cancer Institute. (Washington, DC) V.1-60,
1940-78. For publisher information, see JJIND8. Volume(issue)/page/year:
52,1167,1974
TYPE OF TEST : DNA inhibition
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 100 mg/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 46,305,1977
TYPE OF TEST : Mutation test systems - not otherwise specified
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 100 mg/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 319,19,1993
TYPE OF TEST : Cytogenetic analysis
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 100 mg/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 319,19,1993
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 26 mg/L
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
12(Suppl 13),37,1988
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - hamster Kidney
DOSE/DURATION : 80 ug/L
REFERENCE :
BJCAAI British Journal of Cancer. (Macmillan Press Ltd., Houndmills,
Basingstoke, Hants. RG21 2XS, UK) V.1- 1947- Volume(issue)/page/year:
37,873,1978
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - hamster Liver
DOSE/DURATION : 1 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 136,255,1984
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 450 mg/L
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
10(Suppl 10),1,1987
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 5 mg/L
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
10(Suppl 10),1,1987
TYPE OF TEST : Sister chromatid exchange
ROUTE OF EXPOSURE : Parenteral
TEST SYSTEM : Bird - chicken
DOSE/DURATION : 169 ug/kg
REFERENCE :
47JMAE "Cytogenetic Assays of Environmental Mutagens," Hsu, T.C., ed.,
Totowa, NJ, Allanheld, Osmun & Co., 1982 Volume(issue)/page/year:
-,137,1982
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - rabbit Lymphocyte
DOSE/DURATION : 50 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 89,197,1981
*** REVIEWS ***
ACGIH TLV-Animal carcinogen, NO TWA (skin)
DTLVS* The Threshold Limit Values (TLVs) and Biological Exposure Indices
(BEIs) booklet issues by American Conference of Governmental Industrial
Hygienists (ACGIH), Cincinnati, OH, 1996 Volume(issue)/page/year:
TLV/BEI,1997
IARC Cancer Review:Animal Sufficient Evidence
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 1,87,1972
IARC Cancer Review:Human No Adequate Data
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 1,87,1972
IARC Cancer Review:Group 2B
IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49
Sheridan Ave., Albany, NY 12210) No.1- 1979- Volume(issue)/page/year:
7,56,1987
*** OCCUPATIONAL EXPOSURE LIMITS ***
OEL-AUSTRALIA;Skin;Carcinogen JAN 1993
OEL-FINLAND;Carcinogen JAN 1993
OEL-FRANCE;Carcinogen JAN 1993
OEL-GERMANY;Carcinogen JAN 1993
OEL-SWITZERLAND;Carcinogen JAN 1993
OEL IN BULGARIA, COLOMBIA, JORDAN, KOREA check ACGIH TLV
OEL IN NEW ZEALAND, SINGAPORE, VIETNAM check ACGIH TLV
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH RECOMMENDED EXPOSURE LEVEL (REL) :
NIOSH REL TO o-TOLIDINE-air:CA CL 0.02 mg/m3/60M
REFERENCE :
NIOSH* National Institute for Occupational Safety and Health, U.S. Dept. of
Health, Education, and Welfare, Reports and Memoranda.
Volume(issue)/page/year: DHHS #92-100,1992
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOHS - National Occupational Hazard Survey (1974)
NOHS Hazard Code - 84756
No. of Facilities: 11 (estimated)
No. of Industries: 1
No. of Occupations: 5
No. of Employees: 421 (estimated)
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - 84756
No. of Facilities: 854 (estimated)
No. of Industries: 8
No. of Occupations: 20
No. of Employees: 9640 (estimated)
No. of Female Employees: 6005 (estimated)
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: Carcinogenicity-mouse/rat
EPA GENETOX PROGRAM 1988, Positive: Cell transform.-RLV F344 rat embryo
EPA GENETOX PROGRAM 1988, Positive: Mammalian micronucleus; Histidine
reversion-Ames test
EPA GENETOX PROGRAM 1988, Inconclusive: In vitro UDS-human fibroblast
EPA TSCA Section 8(b) CHEMICAL INVENTORY
On EPA IRIS database
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998
NTP 7th Annual Report on Carcinogens, 1992 : anticipated to be carcinogen
OSHA ANALYTICAL METHOD #ID-71
*** END OF RECORD ***