*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : DD0600000
CHEMICAL NAME : Benzidine, dihydrochloride
CAS REGISTRY NUMBER : 531-85-1
LAST UPDATED : 199710
DATA ITEMS CITED : 31
MOLECULAR FORMULA : C12-H12-N2.2Cl-H
MOLECULAR WEIGHT : 257.18
WISWESSER LINE NOTATION : ZR DR DZ &GH 2
COMPOUND DESCRIPTOR : Tumorigen
Drug
Mutagen
SYNONYMS/TRADE NAMES :
* (1,1'-Biphenyl)-4,4'-diamine, dihydrochloride
* Dihidrocloruro de benzidina
*** HEALTH HAZARD DATA ***
** TUMORIGENIC DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Intraperitoneal
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 62 mg/kg/4W-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Endocrine - tumors
Skin and Appendages - tumors
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 2,747,1981
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 3360 mg/kg/80W-C
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Liver - tumors
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
64,171,1982
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - hamster
DOSE/DURATION : 75 mg/kg/3Y-C
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Liver - tumors
REFERENCE :
85DAAC "Bladder Cancer, A Symposium, Fifth Inter-American Conference on
Toxicology and Occupational Medicine, Coral Gables, FL," Lampe, K.F., et
al., eds., Coral Gables, FL, Aesculapius Pub., 1966 Volume(issue)/page/year:
5,129,1966
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 1600 mg/kg/84W-C
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Liver - tumors
Endocrine - tumors
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
35,2814,1975
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 5040 mg/kg/60W-C
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Liver - tumors
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year:
64,171,1982
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 8064 mg/kg/80W-C
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Lungs, Thorax, or Respiration - tumors
Liver - tumors
REFERENCE :
EJCAAH European Journal of Cancer. (Oxford, UK) V.1-17(6), 1965-1981. For
publisher information, see EJCODS. Volume(issue)/page/year: 16,1205,1980
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 1680 mg/kg/40W-C
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Liver - tumors
REFERENCE :
TOPADD Toxicologic Pathology. (c/o Dr. F.A. de la Iglesia, Warner-Lambert
Co., Pharmaceutical Research Div., POB 1047, Ann Arbor, MI 48106) V.6(3/4)-
1978- Volume(issue)/page/year: 9,1,1981
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 13440 mg/kg/1Y-C
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria
Liver - tumors
REFERENCE :
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E.
First St., Duluth, MN 55802) V.1- 1981- Volume(issue)/page/year:
4,69,1984
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 100 nmol/plate
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 136,33,1984
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 250 ug/well
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 133,161,1984
TYPE OF TEST : DNA adduct
TEST SYSTEM : Bacteria - Escherichia coli
DOSE/DURATION : 20 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 89,95,1981
TYPE OF TEST : DNA adduct
TEST SYSTEM : Human HeLa cell
DOSE/DURATION : 20 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 89,95,1981
TYPE OF TEST : DNA damage
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 250 mg/kg
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
38,1589,1978
TYPE OF TEST : DNA damage
ROUTE OF EXPOSURE : Subcutaneous
TEST SYSTEM : Rodent - rat
DOSE/DURATION : 250 mg/kg
REFERENCE :
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year:
38,1589,1978
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 3 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 216,101,1989
TYPE OF TEST : Micronucleus test
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 300 mg/kg
REFERENCE :
MUTAEX Mutagenesis. (Oxford Univ. Press, Pinkhill House, Southfield Road,
Eynsham, Oxford OX8 1JJ, UK) V.1- 1986- Volume(issue)/page/year: 3,437,1988
TYPE OF TEST : Micronucleus test
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 400 mg/kg/48H (Continuous)
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 234,187,1990
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 30 mg/L
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
12(Suppl 13),37,1988
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - mouse Lymphocyte
DOSE/DURATION : 25 mg/L
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
12(Suppl 13),103,1988
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - hamster Embryo
DOSE/DURATION : 200 umol/L
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For
publisher information, see EMMUEG. Volume(issue)/page/year: 8,515,1986
TYPE OF TEST : Sister chromatid exchange
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - hamster
DOSE/DURATION : 12500 ug/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 113,33,1983
TYPE OF TEST : Sister chromatid exchange
ROUTE OF EXPOSURE : Oral
TEST SYSTEM : Rodent - hamster
DOSE/DURATION : 12500 ug/kg
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 113,33,1983
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 20 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 94,449,1982
*** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA ***
NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA :
NOHS - National Occupational Hazard Survey (1974)
NOHS Hazard Code - 83072
No. of Facilities: 64 (estimated)
No. of Industries: 2
No. of Occupations: 2
No. of Employees: 598 (estimated)
NOES - National Occupational Exposure Survey (1983)
NOES Hazard Code - 83072
No. of Facilities: 103 (estimated)
No. of Industries: 1
No. of Occupations: 2
No. of Employees: 2987 (estimated)
No. of Female Employees: 2464 (estimated)
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: Carcinogenicity-mouse/rat; Cell
transform.-SA7/SHE
EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test
EPA GENETOX PROGRAM 1988, Negative: D melanogaster Sex-linked lethal
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JUNE 1998
*** END OF RECORD ***