*** CHEMICAL IDENTIFICATION ***

RTECS NUMBER            : WH1318000
CHEMICAL NAME           : Spiro(naphthalene-2(1H),2'-oxirane)-3'-carboxaldehyde
                          , 3,5,6,7,8,8a-hexahydro-7-acetoxy-5,6-
                          epoxy-3',8,8a-trimethyl-3-oxo-
CAS REGISTRY NUMBER     : 56299-00-4
LAST UPDATED            : 199410
DATA ITEMS CITED        : 21
MOLECULAR FORMULA       : C17-H20-O6
MOLECULAR WEIGHT        : 320.37
WISWESSER LINE NOTATION : T B366 CO JV KUTJ EOV1 F1 G1 IOV1
COMPOUND DESCRIPTOR     : Tumorigen
                          Mutagen
                          Natural Product
SYNONYMS/TRADE NAMES :
   * PRT
   * PR toxin
   * PR toxine
   * Toxin (Penicillium roquefortii)
   * Toxin PR
 
                       *** HEALTH HAZARD DATA ***
 
                        ** ACUTE TOXICITY DATA **
 
TYPE OF TEST            : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 115 mg/kg
TOXIC EFFECTS :
   Lungs, Thorax, or Respiration - chronic pulmonary edema
   Lungs, Thorax, or Respiration - dyspnea
   Gastrointestinal - other changes
REFERENCE :
   APMBAY Applied Microbiology.  (Washington, DC)  V.1-30, 1953-75.  For
   publisher information, see AEMIDF.  Volume(issue)/page/year: 25,111,1973
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 11600 ug/kg
TOXIC EFFECTS :
   Behavioral - changes in motor activity (specific assay)
   Behavioral - muscle weakness
   Behavioral - ataxia
REFERENCE :
   TOXIA6 Toxicon.  (Pergamon Press Ltd., Headington Hill Hall, Oxford OX3 OBW,
   UK)  V.1-    1962-  Volume(issue)/page/year: 20,433,1982
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 8200 ug/kg
TOXIC EFFECTS :
   Behavioral - changes in motor activity (specific assay)
   Behavioral - muscle weakness
   Behavioral - ataxia
REFERENCE :
   TOXIA6 Toxicon.  (Pergamon Press Ltd., Headington Hill Hall, Oxford OX3 OBW,
   UK)  V.1-    1962-  Volume(issue)/page/year: 20,433,1982
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 72 mg/kg
TOXIC EFFECTS :
   Behavioral - food intake (animal)
   Behavioral - fluid intake
   Behavioral - changes in motor activity (specific assay)
REFERENCE :
   FCTXAV Food and Cosmetics Toxicology.  (London, UK)  V.1-19, 1963-81.  For
   publisher information, see FCTOD7.  Volume(issue)/page/year: 16,369,1978
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 2 mg/kg
TOXIC EFFECTS :
   Behavioral - food intake (animal)
   Behavioral - fluid intake
   Behavioral - changes in motor activity (specific assay)
REFERENCE :
   FCTXAV Food and Cosmetics Toxicology.  (London, UK)  V.1-19, 1963-81.  For
   publisher information, see FCTOD7.  Volume(issue)/page/year: 16,369,1978
 
TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 2 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   85GDA2 "CRC Handbook of Antibiotic Compounds," Vols.1- ,  Berdy, J., Boca
   Raton, FL, CRC Press, 1980-  Volume(issue)/page/year: 6,116,1981
 
                          ** TUMORIGENIC DATA **
 
TYPE OF TEST            : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 100 mg/kg/52D-C
TOXIC EFFECTS :
   Tumorigenic - equivocal tumorigenic agent by RTECS criteria
   Skin and Appendages - tumors
REFERENCE :
   MYCPAH Mycopathologia.  (Kluwer Academic Pub. Group, Distribution Centre,
   POB 322, 3300 AH Dordrecht, Netherlands)  V.1-    1938-
   Volume(issue)/page/year: 78,125,1982
 
TYPE OF TEST            : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 139 mg/kg/52D-C
TOXIC EFFECTS :
   Tumorigenic - equivocal tumorigenic agent by RTECS criteria
   Reproductive - Tumorigenic effects - uterine tumors
REFERENCE :
   MYCPAH Mycopathologia.  (Kluwer Academic Pub. Group, Distribution Centre,
   POB 322, 3300 AH Dordrecht, Netherlands)  V.1-    1938-
   Volume(issue)/page/year: 78,125,1982
 
                            ** MUTATION DATA **
 
TYPE OF TEST            : Mutation in microorganisms
TEST SYSTEM             : Bacteria - Salmonella typhimurium
DOSE/DURATION           : 625 ng/plate
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 130,79,1984
 
TYPE OF TEST            : Mutation in microorganisms
TEST SYSTEM             : Bacteria - Salmonella typhimurium
DOSE/DURATION           : 100 ug/plate
REFERENCE :
   CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
   Sts., Philadelphia, PA 19106)  V.1-    1941-  Volume(issue)/page/year:
   38,536,1978
 
TYPE OF TEST            : DNA repair
TEST SYSTEM             : Bacteria - Bacillus subtilis
DOSE/DURATION           : 100 ug/disc
REFERENCE :
   CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
   Sts., Philadelphia, PA 19106)  V.1-    1941-  Volume(issue)/page/year:
   36,445,1976
 
TYPE OF TEST            : Mutation in microorganisms
TEST SYSTEM             : Mold - Neurospora crassa
DOSE/DURATION           : 2 mg/L
REFERENCE :
   ENMUDM Environmental Mutagenesis.  (New York, NY)  V.1-9, 1979-87.  For
   publisher information, see EMMUEG.  Volume(issue)/page/year: 1,45,1979
 
TYPE OF TEST            : Mutation in microorganisms
TEST SYSTEM             : Yeast - Saccharomyces cerevisiae
DOSE/DURATION           : 30 mg/L
REFERENCE :
   ENMUDM Environmental Mutagenesis.  (New York, NY)  V.1-9, 1979-87.  For
   publisher information, see EMMUEG.  Volume(issue)/page/year: 1,45,1979
 
TYPE OF TEST            : Gene conversion and mitotic recombination
TEST SYSTEM             : Yeast - Saccharomyces cerevisiae
DOSE/DURATION           : 5 mg/L
REFERENCE :
   ENMUDM Environmental Mutagenesis.  (New York, NY)  V.1-9, 1979-87.  For
   publisher information, see EMMUEG.  Volume(issue)/page/year: 1,45,1979
 
TYPE OF TEST            : DNA damage
TEST SYSTEM             : Rodent - rat Liver
DOSE/DURATION           : 25 umol/L
REFERENCE :
   TOERD9 Toxicological European Research.  (S.P.E., 10, Ave. de Messine, 75008
   Paris, France) V.1-    1978-  Volume(issue)/page/year: 2,273,1979
 
TYPE OF TEST            : Mutation test systems - not otherwise specified
ROUTE OF EXPOSURE       : Intraperitoneal
TEST SYSTEM             : Rodent - rat
DOSE/DURATION           : 10 mg/kg
REFERENCE :
   CBINA8 Chemico-Biological Interactions.  (Elsevier Scientific Pub. Ireland
   Ltd., POB 85, Limerick, Ireland)  V.1-    1969-  Volume(issue)/page/year:
   14,207,1976
 
TYPE OF TEST            : Mutation test systems - not otherwise specified
TEST SYSTEM             : Rodent - rat Liver
DOSE/DURATION           : 35 mg/L
REFERENCE :
   CBINA8 Chemico-Biological Interactions.  (Elsevier Scientific Pub. Ireland
   Ltd., POB 85, Limerick, Ireland)  V.1-    1969-  Volume(issue)/page/year:
   18,153,1977

                          *** STATUS IN U.S. ***
 
   EPA GENETOX PROGRAM 1988, Positive: N crassa-reversion; B subtilis rec assay
   
   EPA GENETOX PROGRAM 1988, Positive: S cerevisiae-homozygosis; S
   cerevisiae-reversion
   
   EPA GENETOX PROGRAM 1988, Weakly Positive: S cerevisiae gene conversion
   
   EPA GENETOX PROGRAM 1988, Inconclusive: Histidine reversion-Ames test

 
                            *** END OF RECORD ***