*** CHEMICAL IDENTIFICATION ***
RTECS NUMBER : UU9351900
CHEMICAL NAME : 5H-Pyrido(4,3-b)indole, 3-amino-1,4-dimethyl-
CAS REGISTRY NUMBER : 62450-06-0
BEILSTEIN REFERENCE NO. : 0653052
LAST UPDATED : 199712
DATA ITEMS CITED : 43
MOLECULAR FORMULA : C13-H13-N3
MOLECULAR WEIGHT : 211.29
WISWESSER LINE NOTATION : T B656 DN HMJ C1 EZ F1
COMPOUND DESCRIPTOR : Tumorigen
Mutagen
SYNONYMS/TRADE NAMES :
* 3-Amino-1,4-dimethyl-gamma-carboline
* 3-Amino-1,4-dimethyl-5H-pyrido(4,3-b)indole
* 5H-Pyrido(4,3-b)indol-3-amine, 1,4-dimethyl-
* Trp-P-1
* Trp-P-1
* Tryptophan P1
*** HEALTH HAZARD DATA ***
** ACUTE TOXICITY DATA **
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 100 mg/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold
REFERENCE :
PPTCBY Proceedings of the International Symposium of the Princess Takamatsu
Cancer Research Fund. (Japan Scientific Soc. Press, 6-2-10 Hongo, Bunkyo-ku,
Tokyo 113, Japan) 1st- 1971- Volume(issue)/page/year: 9,159,1979
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 200 mg/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold
REFERENCE :
PPTCBY Proceedings of the International Symposium of the Princess Takamatsu
Cancer Research Fund. (Japan Scientific Soc. Press, 6-2-10 Hongo, Bunkyo-ku,
Tokyo 113, Japan) 1st- 1971- Volume(issue)/page/year: 9,159,1979
TYPE OF TEST : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - hamster
DOSE/DURATION : 380 mg/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold
REFERENCE :
PPTCBY Proceedings of the International Symposium of the Princess Takamatsu
Cancer Research Fund. (Japan Scientific Soc. Press, 6-2-10 Hongo, Bunkyo-ku,
Tokyo 113, Japan) 1st- 1971- Volume(issue)/page/year: 9,159,1979
** TUMORIGENIC DATA **
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 2 gm/kg/49W-C
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Liver - tumors
REFERENCE :
EVHPAZ EHP, Environmental Health Perspectives. (U.S. Government Printing
Office, Supt of Documents, Washington, DC 20402) No.1- 1972-
Volume(issue)/page/year: 67,129,1986
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - rat
DOSE/DURATION : 150 mg/kg/20W-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Endocrine - tumors
Tumorigenic - tumors at site of application
REFERENCE :
PPTCBY Proceedings of the International Symposium of the Princess Takamatsu
Cancer Research Fund. (Japan Scientific Soc. Press, 6-2-10 Hongo, Bunkyo-ku,
Tokyo 113, Japan) 1st- 1971- Volume(issue)/page/year: 9,159,1979
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Oral
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 9648 mg/kg/57W-C
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Liver - tumors
REFERENCE :
EVHPAZ EHP, Environmental Health Perspectives. (U.S. Government Printing
Office, Supt of Documents, Washington, DC 20402) No.1- 1972-
Volume(issue)/page/year: 67,129,1986
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 50 mg/kg
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Blood - lymphoma, including Hodgkin's disease
REFERENCE :
KKZAE5 Kosankinbyo Kenkyusho Zasshi. Journal of the Research Institute of
Tuberculosis and Leprosy. (Tohoku Daigaku Kosankinbyo Kenkyusho Kenkyukai,
4-1 Seiryo-cho, Sendai 980, Japan) V.36- 1984- Volume(issue)/page/year:
37,193,1985
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Implant
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 40 mg/kg
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Kidney, Ureter, Bladder - tumors
REFERENCE :
CALEDQ Cancer Letters (Shannon, Ireland). (Elsevier Scientific Pub. Ireland
Ltd., POB 85, Limerick, Ireland) V.1- 1975- Volume(issue)/page/year:
17,101,1982
TYPE OF TEST : TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - hamster
DOSE/DURATION : 240 mg/kg/20W-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Endocrine - tumors
Tumorigenic - tumors at site of application
REFERENCE :
PPTCBY Proceedings of the International Symposium of the Princess Takamatsu
Cancer Research Fund. (Japan Scientific Soc. Press, 6-2-10 Hongo, Bunkyo-ku,
Tokyo 113, Japan) 1st- 1971- Volume(issue)/page/year: 9,159,1979
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 50 mg/kg
TOXIC EFFECTS :
Tumorigenic - neoplastic by RTECS criteria
Liver - tumors
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 8,1721,1987
TYPE OF TEST : TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE : Subcutaneous
SPECIES OBSERVED : Rodent - mouse
DOSE/DURATION : 50 mg/kg
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria
Blood - lymphoma, including Hodgkin's disease
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 8,1721,1987
** MUTATION DATA **
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 1 ug/plate
REFERENCE :
ABCHA6 Agricultural and Biological Chemistry. (Maruzen Co. Ltd., POB 5050,
Tokyo International, Tokyo 100-31, Japan) V.25- 1961-
Volume(issue)/page/year: 43,1155,1979
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 250 ng/plate
REFERENCE :
JJCREP Japanese Journal of Cancer Research. (Elsevier Science Pub. BV, POB
211, 1000 AE Amsterdam, Netherlands) V.76- 1985-
Volume(issue)/page/year: 76,835,1985
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 10 ug/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 192,239,1987
TYPE OF TEST : Phage inhibition capacity
TEST SYSTEM : Bacteria - Salmonella typhimurium
DOSE/DURATION : 5 umol/plate
REFERENCE :
PPTCBY Proceedings of the International Symposium of the Princess Takamatsu
Cancer Research Fund. (Japan Scientific Soc. Press, 6-2-10 Hongo, Bunkyo-ku,
Tokyo 113, Japan) 1st- 1971- Volume(issue)/page/year: 9,337,1979
TYPE OF TEST : DNA repair
TEST SYSTEM : Bacteria - Bacillus subtilis
DOSE/DURATION : 1200 ng/disc
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 97,339,1982
TYPE OF TEST : DNA damage
TEST SYSTEM : Bacteria - Bacillus subtilis
DOSE/DURATION : 200 ug/disc
REFERENCE :
PMRSDJ Progress in Mutation Research. (Elsevier Science Pub. Co., Inc., 52
Vanderbilt Ave., New York, NY 10017) V.1- 1981- Volume(issue)/page/year:
1,175,1981
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Microorganism - not otherwise specified
DOSE/DURATION : 4 mg/L
REFERENCE :
ABCHA6 Agricultural and Biological Chemistry. (Maruzen Co. Ltd., POB 5050,
Tokyo International, Tokyo 100-31, Japan) V.25- 1961-
Volume(issue)/page/year: 44,1609,1980
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Human Fibroblast
DOSE/DURATION : 20 mg/L
REFERENCE :
JRARAX Journal of Radiation Research. (Japan Radiation Research Soc., c/o
Tokai Daigaku Igakubu Bunshi Seibutsugaku Kyoshitsu, Tenbodai, Isehara
259-11, Japan) V.1- 1960- Volume(issue)/page/year: 24,356,1983
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Human HeLa cell
DOSE/DURATION : 2 umol/L
REFERENCE :
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House,
Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
Volume(issue)/page/year: 13,2389,1992
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Human Fibroblast
DOSE/DURATION : 200 ug/L
REFERENCE :
PJABDW Proceedings of the Japan Academy, Series B: Physical and Biological
Sciences. (Maruzen Co. Ltd., POB 5050, Tokyo International, Tokyo 100-31,
Japan) V.53- 1977- Volume(issue)/page/year: 56,332,1980
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Human Fibroblast
DOSE/DURATION : 200 ug/L
REFERENCE :
PJABDW Proceedings of the Japan Academy, Series B: Physical and Biological
Sciences. (Maruzen Co. Ltd., POB 5050, Tokyo International, Tokyo 100-31,
Japan) V.53- 1977- Volume(issue)/page/year: 56,332,1980
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 100 nmol/L
REFERENCE :
CALEDQ Cancer Letters (Shannon, Ireland). (Elsevier Scientific Pub. Ireland
Ltd., POB 85, Limerick, Ireland) V.1- 1975- Volume(issue)/page/year:
20,283,1983
TYPE OF TEST : DNA inhibition
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 5 mg/L
REFERENCE :
CBINA8 Chemico-Biological Interactions. (Elsevier Scientific Pub. Ireland
Ltd., POB 85, Limerick, Ireland) V.1- 1969- Volume(issue)/page/year:
54,317,1985
TYPE OF TEST : Mutation test systems - not otherwise specified
TEST SYSTEM : Rodent - rat Liver
DOSE/DURATION : 10 mg/L
REFERENCE :
CBINA8 Chemico-Biological Interactions. (Elsevier Scientific Pub. Ireland
Ltd., POB 85, Limerick, Ireland) V.1- 1969- Volume(issue)/page/year:
54,317,1985
TYPE OF TEST : DNA damage
TEST SYSTEM : Rodent - mouse Liver
DOSE/DURATION : 50 umol/L
REFERENCE :
JJCREP Japanese Journal of Cancer Research. (Elsevier Science Pub. BV, POB
211, 1000 AE Amsterdam, Netherlands) V.76- 1985-
Volume(issue)/page/year: 76,835,1985
TYPE OF TEST : DNA damage
ROUTE OF EXPOSURE : Intraperitoneal
TEST SYSTEM : Rodent - mouse
DOSE/DURATION : 10 mg/kg
REFERENCE :
JJCREP Japanese Journal of Cancer Research. (Elsevier Science Pub. BV, POB
211, 1000 AE Amsterdam, Netherlands) V.76- 1985-
Volume(issue)/page/year: 76,835,1985
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - mouse Liver
DOSE/DURATION : 500 nmol/L
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
12,53,1988
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - mouse Mammary gland
DOSE/DURATION : 50 umol/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 261,131,1991
TYPE OF TEST : Mutation in microorganisms
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 1 mg/L
REFERENCE :
BLFSBY Basic Life Sciences. (Plenum Pub. Corp., 223 Spring St., New York,
NY 10003) V.1- 1973- Volume(issue)/page/year: 39,359,1986
TYPE OF TEST : Morphological transformation
TEST SYSTEM : Rodent - hamster Embryo
DOSE/DURATION : 500 ug/L
REFERENCE :
PPTCBY Proceedings of the International Symposium of the Princess Takamatsu
Cancer Research Fund. (Japan Scientific Soc. Press, 6-2-10 Hongo, Bunkyo-ku,
Tokyo 113, Japan) 1st- 1971- Volume(issue)/page/year: 9,151,1979
TYPE OF TEST : Unscheduled DNA synthesis
TEST SYSTEM : Rodent - hamster Liver
DOSE/DURATION : 500 nmol/L
REFERENCE :
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E.
11th St., New York, NY 10003) V.10- 1987- Volume(issue)/page/year:
12,53,1988
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Fibroblast
DOSE/DURATION : 250 ug/L
REFERENCE :
PJABDW Proceedings of the Japan Academy, Series B: Physical and Biological
Sciences. (Maruzen Co. Ltd., POB 5050, Tokyo International, Tokyo 100-31,
Japan) V.53- 1977- Volume(issue)/page/year: 56,332,1980
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 1250 ug/L
REFERENCE :
GMCRDC Gann Monograph on Cancer Research. (Plenum Pub. Corp., 233 Spring
St., New York, NY 10013) No. 11- 1971- Volume(issue)/page/year:
27,95,1981
TYPE OF TEST : Cytogenetic analysis
TEST SYSTEM : Rodent - hamster Ovary
DOSE/DURATION : 25 mg/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE
Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 269,79,1992
TYPE OF TEST : Sister chromatid exchange
TEST SYSTEM : Rodent - hamster Fibroblast
DOSE/DURATION : 250 ug/L
REFERENCE :
PJABDW Proceedings of the Japan Academy, Series B: Physical and Biological
Sciences. (Maruzen Co. Ltd., POB 5050, Tokyo International, Tokyo 100-31,
Japan) V.53- 1977- Volume(issue)/page/year: 56,332,1980
TYPE OF TEST : Mutation in mammalian somatic cells
TEST SYSTEM : Rodent - hamster Lung
DOSE/DURATION : 1 mg/L
REFERENCE :
BLFSBY Basic Life Sciences. (Plenum Pub. Corp., 223 Spring St., New York,
NY 10003) V.1- 1973- Volume(issue)/page/year: 39,359,1986
TYPE OF TEST : DNA adduct
TEST SYSTEM : Mammal - species unspecified Lymphocyte
DOSE/DURATION : 50 umol/L
REFERENCE :
BICHAW Biochemistry. (American Chemical Soc., Distribution Office Dept.
223, POB 57136, West End Stn., Washington, DC 20037) V.1- 1962-
Volume(issue)/page/year: 20,298,1981
*** REVIEWS ***
IARC Cancer Review:Animal Sufficient Evidence
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 31,247,1983
IARC Cancer Review:Human No Adequate Data
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals
to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210)
V.1- 1972- Volume(issue)/page/year: 31,247,1983
IARC Cancer Review:Group 2B
IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49
Sheridan Ave., Albany, NY 12210) No.1- 1979- Volume(issue)/page/year:
7,56,1987
*** STATUS IN U.S. ***
EPA GENETOX PROGRAM 1988, Positive: Carcinogenicity-mouse/rat; SHE-clonal
assay
EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test
*** END OF RECORD ***