Structural Formula Vector Image
Title: FITC
CAS Registry Number: 27072-45-3 (isomeric mixture); 3326-32-7 (5-isothiocyanate); 18861-78-4 (6-isothiocyanate)
CAS Name: 3¢,6¢-Dihydroxy-5(or 6)-isothiocyanatospiro[isobenzofuran-1(3H),9¢-[9H]xanthen]-3-one
Additional Names: fluorescein isothiocyanate
Molecular Formula: C21H11NO5S
Molecular Weight: 389.38
Percent Composition: C 64.78%, H 2.85%, N 3.60%, O 20.54%, S 8.23%
Literature References: Isomeric mixture of the 5- and 6-isothiocyanates; structural analog of the fluorochrome, fluorescein, q.v. Prepn: A. H. Coons et al., J. Immunol. 45, 159 (1942); J. L. Riggs et al., J. Am. Pathol. 34, 1081 (1958). Determn of purity of commerical product: R. M. McKinney et al., Anal. Biochem. 7, 74 (1964); of properites and binding stoichiometry: A. Jobbágy, G. M. Jobbágy, J. Immunol. Methods 2, 159, 169 (1972). Quantitative determn of bound FITC: H. G. de Bruin et al., Vox Sang. 45, 373 (1983). Use as a pH probe: E. Lanz et al., J. Fluorescence 7, 317 (1997); and evaluation of protonation kinetics: J. Widengren et al., Chem. Phys. 249, 259 (1999). Immunofluorescent dye for microscopy: A. Entwistle, M. Noble, J. Microsc. 168, 219 (1992). Label for amino acids: K. Muramoto et al., Meth. Protein Seq. Anal. 1993, 29; F. Dang, Y. Chen, Sci. China B 42, 663 (1999). Detection of anionic detergents: H.-W. Gao, D.-Y. Zhou, Bull. Korean Chem. Soc. 23, 29 (2002).
Properties: Orange solid, dec upon heating.
Use: Fluorescent indicator and probe for biologic systems. Dye for sewage monitoring.

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