Beta Carotene (bay' ta kar' oh teen). ,-Carotene. all-trans--Carotene. (all-E)-1,1¢-(3,7,12,16-Tetramethyl-1,3,5,7,9,11,13,15,17-octadecanonaene-1,18-diyl)bis[2,6,6-trimethylcyclohexene][7235-40-7]. » Beta Carotene contains not less than 96.0 percent and not more than 101.0 percent of C40H56. Packaging and storage Preserve in tight, light-resistant containers. Identification A: Determine the absorbances of Assay preparation B at 455 nm and at 483 nm: the ratio of the absorbance at 455 nm to that at 483 nm is between 1.14 and 1.18. B: Determine the absorbance of Assay preparation B at 455 nm and that of Assay preparation A at 340 nm: the ratio of the absorbance at 455 nm to that at 340 nm is not less than 1.5. Melting range 741: between 176 and 182, with decomposition. Loss on drying 731Dry it in vacuum over phosphorus pentoxide at 40 for 4 hours: it loses not more than 0.2% of its weight. Residue on ignition 281: not more than 0.2%, 2 g of specimen being used. Heavy metals, Method II 231: 0.001%. Assay [notePerform this procedure in subdued light, using low-actinic glassware. ] Assay preparation ATransfer about 50 mg of Beta Carotene, accurately weighed, to a 100-mL volumetric flask, dissolve in 10 mL of acid-free chloroform, dilute with cyclohexane to volume, and mix. Pipet 5 mL of this solution into a 100-mL volumetric flask, dilute with cyclohexane to volume, and mix. Assay preparation BPipet 5 mL of Assay preparation A into a 50-mL volumetric flask, dilute with cyclohexane to volume, and mix. ProcedureDetermine the absorbance of Assay preparation B, at the wavelength of maximum absorbance at about 455 nm, with a suitable spectrophotometer, using cyclohexane as the blank. Calculate the quantity, in mg, of C40H56 in the portion of Beta Carotene taken by the formula: 20,000 AX / 250 in which AX is the absorbance of Assay preparation B; and 250 is the absorptivity of pure beta carotene.Auxiliary InformationPlease check for your question in the FAQs before contacting USP.
USP35NF30 Page 2333 Pharmacopeial Forum: Volume No. 36(6) Page 1498Chromatographic Column Chromatographic columns text is not derived from, and not part of, USP 35 or NF 30. |