Benzyl Benzoate (ben' zil ben' zoe ate). DEFINITION Benzyl Benzoate contains NLT 99.0% and NMT 100.5% of C14H12O2. IDENTIFICATION ASSAY • Procedure Sample: 2 g of Benzyl Benzoate Analysis: Transfer the Sample to a conical flask fitted with a reflux condenser. Add 50.0 mL of 0.5 N alcoholic potassium hydroxide VS, and boil gently for 1 h. Cool, add phenolphthalein TS, and titrate with 0.5 N hydrochloric acid VS. Perform a blank determination (see Titrimetry 541). Each mL of 0.5 N alcoholic potassium hydroxide is equivalent to 106.1 mg of Benzyl Benzoate (C14H12O2). Acceptance criteria: 99.0%100.5% IMPURITIES • Limit of Aldehydes Sample solution: Transfer 10.0 g to a 125-mL conical flask containing 50 mL of alcohol and 5 mL of hydroxylamine hydrochloride solution (3.5 in 100), mix, and allow to stand for 10 min. Analysis: Add 1 mL of bromophenol blue TS, and titrate with 0.1 N sodium hydroxide VS to a light green endpoint. Perform a blank determination, and match the color of the endpoint with that of the titrated Sample solution. Acceptance criteria: The net volume of 0.1 N sodium hydroxide consumed does not exceed 0.50 mL (0.05% as benzaldehyde). SPECIFIC TESTS • Specific Gravity 841: 1.1161.120 • Congealing Temperature 651: Congelation may be brought about by the addition of a fragment of previously congealed Benzyl Benzoate when the temperature has reached the expected congealing temperature. Acceptance criteria: NLT 18.0 • Refractive Index 831: 1.5681.570 at 20 • Acidity: Add 2 drops of phenolphthalein TS to 25 mL of alcohol, and add 0.020 N sodium hydroxide until a pink color is produced. Add 5.0 g of Benzyl Benzoate, and titrate with 0.020 N sodium hydroxide. Acceptance criteria: NMT 1.5 mL of 0.020 N sodium hydroxide is required to restore the pink color. ADDITIONAL REQUIREMENTS • Packaging and Storage: Preserve in tight, well-filled, light-resistant containers, and avoid exposure to excessive heat. Auxiliary Information Please check for your question in the FAQs before contacting USP.
USP35NF30 Page 2331 Chromatographic Column Chromatographic columns text is not derived from, and not part of, USP 35 or NF 30. |