Oleovitamin A and D
(oh'' lee oh vye' ta min).
Oleovitamin A and D is a solution of vitamin A and vitamin D in fish liver oil or in an edible vegetable oil. The vitamin D is present as ergocalciferol or cholecalciferol obtained by the activation of ergosterol or 7-dehydrocholesterol, or from natural sources. Oleovitamin A and D contains NLT 90.0% of the labeled amounts of vitamins A and D.
•  Vitamin A
Analysis: Proceed as directed in Vitamin A Assay 571.
Acceptance criteria: NLT 90.0% of the labeled amount
•  Vitamin D
Analysis: Weigh a quantity of Oleovitamin A and D expected to contain the equivalent of 125–250 µg of vitamin D, but NMT 7.5 mg of vitamin A, and proceed as directed for Chemical Method in Vitamin D Assay 581. If the assay specimen contains less than the equivalent of 2.5 µg/g of vitamin D, or if the ratio of vitamin A to vitamin D exceeds 300:1, proceed as directed for Biological Method in Vitamin D Assay 581.
Acceptance criteria: NLT 90.0% of the labeled amount
•  Packaging and Storage: Preserve in tight containers, protected from light and air, preferably under an atmosphere of an inert gas. Store in a dry place.
•  Labeling: Label it to indicate the content of vitamin A in terms of retinol per g. The vitamin A content may also be expressed in USP Vitamin A Units per g. Label it to show whether it contains ergocalciferol, cholecalciferol, or vitamin D from a natural source. Label it to indicate also the vitamin D content, in µg, of ergocalciferol or cholecalciferol per g. Its vitamin D content may be expressed also in USP Vitamin D Units per g.
•  USP Reference Standards 11
USP Cholecalciferol RS Click to View Structure
USP Ergocalciferol RS Click to View Structure
Auxiliary Information— Please check for your question in the FAQs before contacting USP.
Topic/QuestionContactExpert Committee
MonographHuy T. Dinh, M.S.
Scientific Liaison
(DS2010) Monographs - Dietary Supplements
Reference StandardsRS Technical Services
USP35–NF30 Page 4109
Chromatographic Column— 
Chromatographic columns text is not derived from, and not part of, USP 35 or NF 30.