Oleovitamin A and D
(oh'' lee oh vye' ta min).
DEFINITION
Oleovitamin A and D is a solution of vitamin A and vitamin D in fish liver oil or in an edible vegetable oil. The vitamin D is present as ergocalciferol or cholecalciferol obtained by the activation of ergosterol or 7-dehydrocholesterol, or from natural sources. Oleovitamin A and D contains NLT 90.0% of the labeled amounts of vitamins A and D.
ASSAY
• Vitamin A
Analysis: Proceed as directed in Vitamin A Assay 
571
.
571.Acceptance criteria: NLT 90.0% of the labeled amount
• Vitamin D
Analysis: Weigh a quantity of Oleovitamin A and D expected to contain the equivalent of 125–250 µg of vitamin D, but NMT 7.5 mg of vitamin A, and proceed as directed for Chemical Method in Vitamin D Assay 581. If the assay specimen contains less than the equivalent of 2.5 µg/g of vitamin D, or if the ratio of vitamin A to vitamin D exceeds 300:1, proceed as directed for Biological Method in Vitamin D Assay 581.
581. If the assay specimen contains less than the equivalent of 2.5 µg/g of vitamin D, or if the ratio of vitamin A to vitamin D exceeds 300:1, proceed as directed for Biological Method in Vitamin D Assay 581.Acceptance criteria: NLT 90.0% of the labeled amount
ADDITIONAL REQUIREMENTS
• Packaging and Storage: Preserve in tight containers, protected from light and air, preferably under an atmosphere of an inert gas. Store in a dry place.
• Labeling: Label it to indicate the content of vitamin A in terms of retinol per g. The vitamin A content may also be expressed in USP Vitamin A Units per g. Label it to show whether it contains ergocalciferol, cholecalciferol, or vitamin D from a natural source. Label it to indicate also the vitamin D content, in µg, of ergocalciferol or cholecalciferol per g. Its vitamin D content may be expressed also in USP Vitamin D Units per g.
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Auxiliary Information— Please check for your question in the FAQs before contacting USP.
| Topic/Question | Contact | Expert Committee |
|---|---|---|
| Monograph | Huy T. Dinh, M.S. Scientific Liaison 1-301-816-8594 | (DS2010) Monographs - Dietary Supplements |
| Reference Standards | RS Technical Services 1-301-816-8129 rstech@usp.org |
USP35–NF30 Page 4109
Chromatographic Column—
Chromatographic columns text is not derived from, and not part of, USP 35 or NF 30.