Neostigmine Bromide
(nee'' oh stig' meen broe' mide).
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303.20Benzenaminium, 3-[[(dimethylamino)carbonyl]oxy]-N,N,N-trimethyl-, bromide.
(m-Hydroxyphenyl)trimethylammonium bromide dimethylcarbamate
[114-80-7].» Neostigmine Bromide contains not less than 98.0 percent and not more than 102.0 percent of C12H19BrN2O2, calculated on the dried basis.
Packaging and storage—
Preserve in tight containers.
USP Reference standards 
11
—
11—
USP Neostigmine Bromide RS 
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Identification—
B:
A solution (1 in 50) responds to the tests for Bromide 191.
191.
Melting range 741:
between 171
and 176, with decomposition.
741:
between 171 and 176, with decomposition.
Loss on drying 731—
Dry it at 105 for 3 hours: it loses not more than 2.0% of its weight.
731—
Dry it at 105 for 3 hours: it loses not more than 2.0% of its weight.
Residue on ignition 281:
not more than 0.15%.
281:
not more than 0.15%.
Sulfate—
Dissolve 250 mg in 10 mL of water, and add 1 mL of 3 N hydrochloric acid and 1 mL of barium chloride TS: no turbidity is produced immediately.
Assay—
Dissolve about 750 mg of Neostigmine Bromide, accurately weighed, in a mixture of 70 mL of glacial acetic acid and 20 mL of mercuric acetate TS, add 4 drops of crystal violet TS, and titrate with 0.1 N perchloric acid VS to a blue endpoint. Perform a blank determination, and make any necessary correction. Each mL of 0.1 N perchloric acid is equivalent to 30.32 mg of C12H19BrN2O2.
Auxiliary Information—
Please check for your question in the FAQs before contacting USP.
| Topic/Question | Contact | Expert Committee |
|---|---|---|
| Monograph | Hariram Ramanathan, M.S.
Associate Scientific Liaison 1-301-816-8313 |
(SM42010) Monographs - Small Molecules 4 |
| Reference Standards | RS Technical Services 1-301-816-8129 rstech@usp.org |
USP35–NF30 Page 4034