Methacholine Chloride
(meth'' a koe' leen klor' ide).
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195.691-Propanaminium, 2-(acetyloxy)-N,N,N-trimethyl-, chloride, (±)-.
(±)-(2-Hydroxypropyl)trimethylammonium chloride acetate
[62-51-1].» Methacholine Chloride, dried at 105
for 4 hours, contains not less than 98.0 percent and not more than 101.0 percent of C8H18ClNO2.
for 4 hours, contains not less than 98.0 percent and not more than 101.0 percent of C8H18ClNO2.
Packaging and storage—
Preserve in tight containers.
Identification—
A:
Infrared Absorption 
197M
.
197M.
B:
A solution (1 in 50) responds to the tests for Chloride 191.
191.
Loss on drying 731—
Dry it at 105 for 4 hours: it loses not more than 1.5% of its weight.
731—
Dry it at 105 for 4 hours: it loses not more than 1.5% of its weight.
Residue on ignition 281:
not more than 0.1%.
281:
not more than 0.1%.
Acetylcholine chloride—
To 2 mL of a solution (1 in 10) add 3 mL of a solution of sodium perchlorate (1 in 5), shake, and immerse in ice water for 5 minutes: no precipitate is formed.
Heavy metals, Method II 231:
0.002%.
231:
0.002%.
Assay—
Transfer to a conical flask about 400 mg of Methacholine Chloride, previously dried and accurately weighed (because it is very hygroscopic, store the dried material in a vacuum desiccator), dissolve it in 50 mL of glacial acetic acid, add 10 mL of mercuric acetate TS and 1 drop of crystal violet TS, and titrate with 0.1 N perchloric acid VS to a blue-green endpoint. Perform a blank determination, and make any necessary correction. Each mL of 0.1 N perchloric acid is equivalent to 19.57 mg of C8H18ClNO2.
Auxiliary Information—
Please check for your question in the FAQs before contacting USP.
| Topic/Question | Contact | Expert Committee |
|---|---|---|
| Monograph | Hariram Ramanathan, M.S.
Associate Scientific Liaison 1-301-816-8313 |
(SM42010) Monographs - Small Molecules 4 |
USP35–NF30 Page 3837
Pharmacopeial Forum: Volume No. 34(3) Page 629