Glycine
(glye' seen).

C2H5NO2 75.07
Glycine     [56-40-6].
DEFINITION
Glycine contains NLT 98.5% and NMT 101.5% of glycine (C2H5NO2), calculated on the dried basis.
IDENTIFICATION
ASSAY
•  Procedure
Sample:  150 mg of Glycine
Blank:  100 mL of glacial acetic acid
Titrimetric system
Mode:  Direct titration
Titrant:  0.1 N perchloric acid VS
Endpoint detection:  Visual
Analysis:  Dissolve the Sample in 100 mL of glacial acetic acid, and add 1 drop of crystal violet TS. Titrate with the Titrant to a green endpoint. Perform the Blank determination.
Calculate the percentage of glycine (C2H5NO2) in the Sample taken:
Result = {[(VS VB) × N × F]/W} × 100
 VS = = Titrant volume consumed by the Sample (mL) VB = = Titrant volume consumed by the Blank (mL) N = = actual normality of the Titrant (mEq/mL) F = = equivalency factor, 75.07 mg/mEq W = = Sample weight (mg)
Acceptance criteria:  98.5%–101.5% on the dried basis
IMPURITIES
NMT 0.1%
Standard solution:  0.10 mL of 0.020 N hydrochloric acid
Sample:  1 g of Glycine
Acceptance criteria:  NMT 0.007%
Standard solution:  0.20 mL of 0.020 N sulfuric acid
Sample:  3 g of Glycine
Acceptance criteria:  NMT 0.0065%
NMT 20 ppm
•  Hydrolyzable Substances
Sample solution:  100 mg/mL of Glycine
Analysis:  Boil 10 mL of the Sample solution for 1 min, and set aside for 2 h.
Acceptance criteria:  The solution appears as clear and as mobile as 10 mL of the same solution that has not been boiled.
SPECIFIC TESTS
Dry a sample at 105 for 2 h: it loses NMT 0.2% of its weight.