l-Glutamic Acid, Hydrochloride
l-2-Aminoglutaric acid, hydrochloride;
2-Aminopentanedioic acid, hydrochloride [138-15-8].
l-Glutamic Acid, Hydrochloride, contains NLT 98.5% and NMT 101.5% of C5H9NO4·HCl, calculated on the dried basis.
Sample: 100 mg of l-Glutamic Acid, Hydrochloride, previously dried
Analysis: Dissolve the Sample in 0.5 mL of water, add 15.0 mL of 0.1 N perchloric acid VS, and heat on a water bath for 30 min. After cooling, add 45 mL of glacial acetic acid, and titrate the excess perchloric acid with 0.1 N sodium acetate, determining the endpoint potentiometrically. Perform a blank determination (see Titrimetry 541). Each mL of 0.1 N perchloric acid is equivalent to 18.36 mg of C5H9NO4·HCl.
Acceptance criteria: 98.5%101.5% on the dried basis
• Optical Rotation, Specific Rotation 781S: +25.2 to +25.8, determined at 20
Sample solution: 100 mg/mL, in 2 N hydrochloric acid
• Loss on Drying 731: Dry a sample at 80 for 4 h: it loses NMT 0.5% of its weight.
• Packaging and Storage: Store in well-closed, light-resistant containers.
• USP Reference Standards 11
USP l-Glutamic Acid, Hydrochloride RS
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USP35NF30 Page 1810Pharmacopeial Forum: Volume No. 35(5) Page 1203