Clioquinol Cream
» Clioquinol Cream contains not less than 90.0 percent and not more than 110.0 percent of the labeled amount of C9H5ClINO in a suitable cream base.
Packaging and storage—
Preserve in collapsible tubes or tight, light-resistant containers.
USP Reference standards 
11
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11—
USP Clioquinol RS 
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Identification—
A:
Prepare a Standard solution as directed for Standard preparation in the Assay, except to use 1.0 mL of pyridine instead of the Internal standard solution, and chromatograph as directed in the Assay: the chromatogram of the Assay preparation obtained in the Assay exhibits a peak for clioquinol, the retention time of which corresponds with that exhibited by the Standard solution.
B:
Place a quantity of Cream, equivalent to about 25 mg of clioquinol, in a 100-mL volumetric flask, add about 75 mL of dilute hydrochloric acid (1 in 4), and heat on a steam bath to melt the cream, shaking vigorously to extract the clioquinol. Cool under running water, and add dilute hydrochloric acid (1 in 4) to volume. Filter through paper, and dilute 3 mL of the filtrate with dilute hydrochloric acid (1 in 4) to 100 mL: the UV absorption spectrum of this solution exhibits maxima and minima at the same wavelengths as that of a similar solution of USP Clioquinol RS, concomitantly measured.
Assay—
Internal standard solution, Standard preparation, and Chromatographic system—
Proceed as directed in the Assay under Clioquinol.
Assay preparation—
Transfer an accurately weighed portion of Cream, equivalent to about 150 mg of clioquinol, to a 60-mL separator. Place the separator on its side in a vacuum oven at a pressure of about 10 mm of mercury at 45
for 4 hours. Remove the separator from the oven, allow to cool, add 15 mL of a mixture of pyridine and n-hexane (4:1), insert a polytef stopper, and mix. Transfer the mixture to a 50-mL volumetric flask, and rinse the separator with two 15-mL portions of the same solvent, shaking each time for 30 seconds. Transfer both rinsings to the volumetric flask, dilute with the same solvent to volume, and mix. Transfer 1.0 mL to a screw-capped glass vial fitted with a septum, add 1.0 mL of bis(trimethylsilyl)acetamide and 1.0 mL of Internal standard solution, attach the cap, and mix. Heat in a water bath at 50 for 15 minutes, and then cool to ambient temperature.
for 4 hours. Remove the separator from the oven, allow to cool, add 15 mL of a mixture of pyridine and n-hexane (4:1), insert a polytef stopper, and mix. Transfer the mixture to a 50-mL volumetric flask, and rinse the separator with two 15-mL portions of the same solvent, shaking each time for 30 seconds. Transfer both rinsings to the volumetric flask, dilute with the same solvent to volume, and mix. Transfer 1.0 mL to a screw-capped glass vial fitted with a septum, add 1.0 mL of bis(trimethylsilyl)acetamide and 1.0 mL of Internal standard solution, attach the cap, and mix. Heat in a water bath at 50 for 15 minutes, and then cool to ambient temperature.
Procedure—
Proceed as directed for Procedure in the Assay under Clioquinol. Calculate the quantity, in mg, of C9H5ClINO in the portion of Cream taken by the formula:
50C(RU / RS)
in which C is the concentration, in mg per mL, of USP Clioquinol RS in the Standard solution used to prepare the Standard preparation; and RU and RS are the ratios of the peak responses of the clioquinol peak to the internal standard peak obtained from the Assay preparation and the Standard preparation, respectively.
Auxiliary Information—
Please check for your question in the FAQs before contacting USP.
| Topic/Question | Contact | Expert Committee |
|---|---|---|
| Monograph | Behnam Davani, Ph.D., M.B.A.
Senior Scientific Liaison 1-301-816-8394 |
(SM12010) Monographs - Small Molecules 1 |
| Reference Standards | RS Technical Services 1-301-816-8129 rstech@usp.org |
USP35–NF30 Page 2710