Biotin
244.311H-Thieno3,4-dimidazole-4-pentanoic acid, hexahydro-2-oxo-, 3aS-(3a
,4
,6a)-.
(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno3,4-dimidazole-4-valeric acid
[58-85-5].» Biotin contains not less than 97.5 percent and not more than 100.5 percent of C10H16N2O3S.
Packaging and storage—
Store in tight containers.
Specific rotation 
781S
:
between +89
and +93.
781S:
between +89 and +93.
Test solution:
20 mg per mL, in 0.1 N sodium hydroxide.
Assay—
Mix about 500 mg, accurately weighed, of Biotin with 100 mL of water, add phenolphthalein TS, and titrate the suspension slowly with 0.1 N sodium hydroxide VS, while heating and stirring continuously. Each mL of 0.1 N sodium hydroxide is equivalent to 24.43 mg of C10H16N2O3S.
Auxiliary Information—
Please check for your question in the FAQs before contacting USP.
Chromatographic Column—
| Topic/Question | Contact | Expert Committee |
| Monograph | Curtis Phinney
1-301-816-8540 |
(DSN05) Dietary Supplements - Non-Botanicals |
| Reference Standards | Lili Wang, Technical Services Scientist 1-301-816-8129 RSTech@usp.org |
USP32–NF27 Page 1679
Chromatographic columns text is not derived from, and not part of, USP 32 or NF 27.