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C10H16N2O3S 244.31

1H-Thieno3,4-dimidazole-4-pentanoic acid, hexahydro-2-oxo-, 3aS-(3a,4,6a)-.
(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno3,4-dimidazole-4-valeric acid [58-85-5].
» Biotin contains not less than 97.5 percent and not more than 100.5 percent of C10H16N2O3S.
Packaging and storage— Store in tight containers.
Specific rotation 781S: between +89 and +93.
Test solution: 20 mg per mL, in 0.1 N sodium hydroxide.
Assay— Mix about 500 mg, accurately weighed, of Biotin with 100 mL of water, add phenolphthalein TS, and titrate the suspension slowly with 0.1 N sodium hydroxide VS, while heating and stirring continuously. Each mL of 0.1 N sodium hydroxide is equivalent to 24.43 mg of C10H16N2O3S.
Auxiliary Information— Please check for your question in the FAQs before contacting USP.
Topic/Question Contact Expert Committee
Monograph Curtis Phinney

(DSN05) Dietary Supplements - Non-Botanicals
Reference Standards Lili Wang, Technical Services Scientist
USP32–NF27 Page 1679
Chromatographic Column—
Chromatographic columns text is not derived from, and not part of, USP 32 or NF 27.