1H-Thieno3,4-dimidazole-4-pentanoic acid, hexahydro-2-oxo-, 3aS-(3a,4,6a)-.
(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno3,4-dimidazole-4-valeric acid [58-85-5].
» Biotin contains not less than 97.5 percent and not more than 100.5 percent of C10H16N2O3S.
Packaging and storage Store in tight containers.
Specific rotation 781S: between +89 and +93.
Test solution: 20 mg per mL, in 0.1 N sodium hydroxide.
Assay Mix about 500 mg, accurately weighed, of Biotin with 100 mL of water, add phenolphthalein TS, and titrate the suspension slowly with 0.1 N sodium hydroxide VS, while heating and stirring continuously. Each mL of 0.1 N sodium hydroxide is equivalent to 24.43 mg of C10H16N2O3S.
Auxiliary Information Please check for your question in the FAQs before contacting USP.Chromatographic Column
USP32NF27 Page 1679
Chromatographic columns text is not derived from, and not part of, USP 32 or NF 27.