Vanillin
152.15Benzaldehyde, 4-hydroxy-3-methoxy-.
Vanillin
[121-33-5].» Vanillin contains not less than 97.0 percent and not more than 103.0 percent of C8H8O3, calculated on the dried basis.
Packaging and storage—
Preserve in tight, light-resistant containers.
Identification—
and 83
Loss on drying 
731
—
Dry it over silica gel for 4 hours: it loses not more than 1.0% of its weight.
731—
Dry it over silica gel for 4 hours: it loses not more than 1.0% of its weight.
Residue on ignition 281:
not more than 0.05%.
281:
not more than 0.05%.
Assay—
Transfer about 100 mg of Vanillin, accurately weighed, to a 250-mL volumetric flask, add methanol to volume, and mix. Pipet 2 mL of this solution into a 100-mL volumetric flask, add methanol to volume, and mix. Dissolve an accurately weighed quantity of USP Vanillin RS in methanol, and dilute quantitatively and stepwise with methanol to obtain a Standard solution having a known concentration of about 8 µg per mL. Concomitantly determine the absorbances of both solutions in 1-cm cells at the wavelength of maximum absorbance at about 308 nm, with a suitable spectrophotometer, using methanol as the blank. Calculate the quantity, in mg, of C8H8O3 in the portion of Vanillin taken by the formula:
12.5C( A U / A S )
in which C is the concentration, in µg per mL, of USP Vanillin RS in the Standard solution; and A U and A S are the absorbances of the solution of Vanillin and the Standard solution, respectively.
Auxiliary Information—
Please check for your question in the FAQs before contacting USP.
Chromatographic Column—
| Topic/Question | Contact | Expert Committee |
| Monograph | Robert H. Lafaver, B.A.
Scientist 1-301-816-8335 |
(EM105) Excipient Monographs 1 |
| Reference Standards | Lili Wang, Technical Services Scientist 1-301-816-8129 RSTech@usp.org |
USP32–NF27 Page 1375
Chromatographic columns text is not derived from, and not part of, USP 32 or NF 27.