» Vanillin contains not less than 97.0 percent and not more than 103.0 percent of C8H8O3, calculated on the dried basis.
Packaging and storage Preserve in tight, light-resistant containers.
B: Ultraviolet Absorption 197U
Solution: 8 µg per mL.
Melting range 741: between 81 and 83.
Loss on drying 731 Dry it over silica gel for 4 hours: it loses not more than 1.0% of its weight.
Residue on ignition 281: not more than 0.05%.
Assay Transfer about 100 mg of Vanillin, accurately weighed, to a 250-mL volumetric flask, add methanol to volume, and mix. Pipet 2 mL of this solution into a 100-mL volumetric flask, add methanol to volume, and mix. Dissolve an accurately weighed quantity of USP Vanillin RS in methanol, and dilute quantitatively and stepwise with methanol to obtain a Standard solution having a known concentration of about 8 µg per mL. Concomitantly determine the absorbances of both solutions in 1-cm cells at the wavelength of maximum absorbance at about 308 nm, with a suitable spectrophotometer, using methanol as the blank. Calculate the quantity, in mg, of C8H8O3 in the portion of Vanillin taken by the formula:
12.5C( A U / A S )in which C is the concentration, in µg per mL, of USP Vanillin RS in the Standard solution; and A U and A S are the absorbances of the solution of Vanillin and the Standard solution, respectively.
Auxiliary Information Please check for your question in the FAQs before contacting USP.Chromatographic Column
USP32NF27 Page 1375
Chromatographic columns text is not derived from, and not part of, USP 32 or NF 27.