Betaine Hydrochloride
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C5H11NO2·HCl 153.61

Methanaminium, 1-carboxy-N, N, N-trimethyl-, chloride.
Betaine hydrochloride.
(Carboxymethyl)trimethylammonium chloride [590-46-5].
» Betaine Hydrochloride contains not less than 98.0 percent and not more than 100.5 percent of C5 H11NO2·HCl, calculated on the anhydrous basis.
Packaging and storage— Preserve in well-closed containers.
B: A solution (1 in 20) responds to the tests for Chloride 191.
pH 791: between 0.8 and 1.2, in a solution (1 in 4).
Water, Method I 921: not more than 0.5%.
Residue on ignition 281: not more than 0.1%.
Heavy metals 231: 0.001%.
Assay— Transfer about 400 mg of Betaine Hydrochloride, accurately weighed, to a conical flask, add 50 mL of glacial acetic acid, and heat gently with swirling until solution is complete. Add 25 mL of mercuric acetate TS, cool, add 2 drops of crystal violet TS, and titrate with 0.1 N perchloric acid VS to a green endpoint. Perform a blank determination, and make any necessary correction. Each mL of 0.1 N perchloric acid is equivalent to 15.36 mg of C5H11NO2·HCl.
Auxiliary Information— Please check for your question in the FAQs before contacting USP.
Topic/Question Contact Expert Committee
Monograph Elena Gonikberg, Ph.D.
Senior Scientist
(MDGRE05) Monograph Development-Gastrointestinal Renal and Endocrine
Reference Standards Lili Wang, Technical Services Scientist
USP32–NF27 Page 1655