» Beta Carotene contains not less than 96.0 percent and not more than 101.0 percent of C40H56.
Packaging and storage Preserve in tight, light-resistant containers.
A: Determine the absorbances of Assay preparation B at 455 nm and at 483 nm: the ratio of the absorbance at 455 nm to that at 483 nm is between 1.14 and 1.18.
B: Determine the absorbance of Assay preparation B at 455 nm and that of Assay preparation A at 340 nm: the ratio of the absorbance at 455 nm to that at 340 nm is not less than 1.5.
Melting range 741: between 176 and 182, with decomposition.
Loss on drying 731 Dry it in vacuum over phosphorus pentoxide at 40 for 4 hours: it loses not more than 0.2% of its weight.
Residue on ignition 281: not more than 0.2%, 2 g of specimen being used.
Heavy metals, Method II 231: 0.001%.
Assay [notePerform this procedure in subdued light, using low-actinic glassware.]
Assay preparation A Transfer about 50 mg of Beta Carotene, accurately weighed, to a 100-mL volumetric flask, dissolve in 10 mL of acid-free chloroform, dilute with cyclohexane to volume, and mix. Pipet 5 mL of this solution into a 100-mL volumetric flask, dilute with cyclohexane to volume, and mix.
Assay preparation B Pipet 5 mL of Assay preparation A into a 50-mL volumetric flask, dilute with cyclohexane to volume, and mix.
Procedure Determine the absorbance of Assay preparation B, at the wavelength of maximum absorbance at about 455 nm, with a suitable spectrophotometer, using cyclohexane as the blank. Calculate the quantity, in mg, of C40H56 in the portion of Beta Carotene taken by the formula:
20,000 AX / 250in which AX is the absorbance of Assay preparation B; and 250 is the absorptivity of pure beta carotene.
Auxiliary Information Please check for your question in the FAQs before contacting USP.Chromatographic Column
USP32NF27 Page 1654
Chromatographic columns text is not derived from, and not part of, USP 32 or NF 27.