Sulfapyridine
249.29Benzenesulfonamide, 4-amino-N-2-pyridinyl-.
N1-2-Pyridylsulfanilamide
[144-83-2].» Sulfapyridine contains not less than 99.0 percent and not more than 100.5 percent of C11H11N3O2S, calculated on the dried basis.
Packaging and storage—
Preserve in well-closed, light-resistant containers.
Clarity and color of solution—
A solution of 1.0 g in a mixture of 20 mL of water and 5 mL of 1 N sodium hydroxide is clear and not more deeply colored than pale yellow.
Identification—
B:
Add 5 mL of 3 N hydrochloric acid to about 0.1 g of Sulfapyridine, and boil gently for about 5 minutes. Cool in an ice bath, add 4 mL of sodium nitrite solution (1 in 100), dilute with water to 10 mL, and place the mixture in the ice bath for 10 minutes. To 5 mL of the cooled mixture add a solution of 50 mg of 2-naphthol in 2 mL of sodium hydroxide solution (1 in 10): an orange-red precipitate is formed, and it darkens on standing.
Acidity—
Digest 2.0 g with 100 mL of water at about 70
for 5 minutes, cool at once to about 20, and filter. To 25.0 mL of the filtrate add 2 drops of phenolphthalein TS, and titrate with 0.10 N sodium hydroxide: not more than 0.20 mL is required for neutralization.
for 5 minutes, cool at once to about 20, and filter. To 25.0 mL of the filtrate add 2 drops of phenolphthalein TS, and titrate with 0.10 N sodium hydroxide: not more than 0.20 mL is required for neutralization.
Loss on drying 
731
—
Dry it at 105 for 2 hours: it loses not more than 0.5% of its weight.
731—
Dry it at 105 for 2 hours: it loses not more than 0.5% of its weight.
Residue on ignition 281:
not more than 0.1%.
281:
not more than 0.1%.
Selenium 291:
0.003%, a 200-mg test specimen being used.
291:
0.003%, a 200-mg test specimen being used.
Heavy metals, Method II 231:
0.002%.
231:
0.002%.
Assay—
Proceed with Sulfapyridine as directed under Nitrite Titration 451. Each mL of 0.1 N sodium nitrite is equivalent to 24.93 mg of C11H11N3O2S.
451. Each mL of 0.1 N sodium nitrite is equivalent to 24.93 mg of C11H11N3O2S.
Auxiliary Information—
Please check for your question in the FAQs before contacting USP.
Chromatographic Column—
| Topic/Question | Contact | Expert Committee |
| Monograph | Behnam Davani, Ph.D., M.B.A.
Senior Scientist 1-301-816-8394 |
(MDAA05) Monograph Development-Antivirals and Antimicrobials |
| Reference Standards | Lili Wang, Technical Services Scientist 1-301-816-8129 RSTech@usp.org |
USP32–NF27 Page 3630
Chromatographic columns text is not derived from, and not part of, USP 32 or NF 27.