Ascorbic Acid
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C6H8O6 176.12

l-Ascorbic acid.
l-Ascorbic acid [50-81-7].
» Ascorbic Acid contains not less than 99.0 percent and not more than 100.5 percent of C6H8O6.
Packaging and storage— Preserve in tight, light-resistant containers.
B: A solution (1 in 50) reduces alkaline cupric tartrate TS slowly at room temperature but more readily upon heating.
Specific rotation 781S: between +20.5 and +21.5, the optical rotation being measured immediately, following the preparation of the solution.
Test solution: 100 mg per mL, in carbon dioxide-free water.
Residue on ignition 281: not more than 0.1%.
Heavy metals 231 Dissolve 1 g in 25 mL of water: the limit is 0.002%.
Assay— Dissolve about 400 mg of Ascorbic Acid, accurately weighed, in a mixture of 100 mL of water and 25 mL of 2 N sulfuric acid. Add 3 mL of starch TS, and titrate at once with 0.1 N iodine VS. Each mL of 0.1 N iodine is equivalent to 8.806 mg of C6H8O6.
Auxiliary Information— Please check for your question in the FAQs before contacting USP.
Topic/Question Contact Expert Committee
Monograph Curtis Phinney

(DSN05) Dietary Supplements - Non-Botanicals
Reference Standards Lili Wang, Technical Services Scientist
USP32–NF27 Page 1580
Chromatographic Column—
Chromatographic columns text is not derived from, and not part of, USP 32 or NF 27.