Acetohexamide
Click to View Image
C15H20N2O4S 324.40

Benzenesulfonamide, 4-acetyl-N-[[cyclohexylamino]carbonyl]-.
1-[(p-Acetylphenyl)sulfonyl]-3-cyclohexylurea [968-81-0].
» Acetohexamide contains not less than 97.0 percent and not more than 101.0 percent of C15H20N2O4S, calculated on the dried basis.
Packaging and storage— Preserve in well-closed containers.
Identification—
B: Ultraviolet Absorption 197U
Solution: 10 µg per mL.
Medium: 0.01 N sodium hydroxide.
Absorptivities at 247 nm, calculated on the dried basis, do not differ by more than 3.0%.
Melting range 741: between 182.5 and 187.
Loss on drying 731 Dry it at 105 for 3 hours: it loses not more than 1.0% of its weight.
Residue on ignition 281: not more than 0.1%.
Selenium 291: 0.003%, a 200-mg specimen mixed with 200 mg of magnesium oxide being used.
Assay— Dissolve about 300 mg of Acetohexamide, accurately weighed, in 40 mL of dimethylformamide, add 5 drops of thymol blue TS, and titrate, using a magnetic stirrer, with 0.1 N sodium methoxide VS to a blue endpoint. Perform a blank determination, and make any necessary correction. Each mL of 0.1 N sodium methoxide is equivalent to 32.44 mg of C15H20N2O4S.
Auxiliary Information— Please check for your question in the FAQs before contacting USP.
Topic/Question Contact Expert Committee
Monograph Elena Gonikberg, Ph.D.
Senior Scientist
1-301-816-8251
(MDGRE05) Monograph Development-Gastrointestinal Renal and Endocrine
Reference Standards Lili Wang, Technical Services Scientist
1-301-816-8129
RSTech@usp.org
USP32–NF27 Page 1420
Pharmacopeial Forum: Volume No. 27(3) Page 2501
Chromatographic Column—
Chromatographic columns text is not derived from, and not part of, USP 32 or NF 27.