A: Transfer a volume of Injection, equivalent to about 100 mg of menadiol sodium diphosphate, to a separator, add 10 mL of 2 N sulfuric acid, and extract with six 25-mL portions of ether, discarding the ether extracts. To the aqueous solution add 1 mL of 0.5 N ceric sulfate and 1 mL of 30 percent hydrogen peroxide, and extract with two 10-mL portions of chloroform. Evaporate the combined chloroform extracts on a steam bath just to dryness, then dry at 80 for 1 hour: the IR absorption spectrum of a potassium bromide dispersion of the menadione so obtained exhibits maxima at the same wavelengths as that of a similar preparation of USP Menadione RS. The solid also responds to Identification test B under Menadiol Sodium Diphosphate.

B: Adjust, if necessary, a volume of Injection, equivalent to about 20 mg of menadiol sodium diphosphate, by evaporation or dilution with water, as required, to 2 mL: the solution responds to Identification test C under Menadiol Sodium Diphosphate.