Powdered Licorice Extract
» Powdered Licorice Extract is prepared from comminuted Licorice extracted with water or suitable solvents such as alcohol, water, or mixtures of these solvents. The ratio of the crude plant material to Powdered Extract is between 5:1 and 7:1. It contains not less than 6.0 percent of glycyrrhizic acid (C42H62O16), calculated on the dried basis.
Packaging and storage— Preserve in tight containers, protected from light.
Labeling— The label states the Latin binomial and, following the official name, the part of the plant from which the article was derived. The label also indicates the content of glycyrrhizic acid, the extracting solvent or solvent mixture used for preparation, and the ratio of the starting material to final product. The label bears a statement indicating that “Excessive amounts or long-term use of Licorice may cause high blood pressure or low potassium, which have been associated with irregular heartbeat and/or muscle weakness. Licorice may worsen the effects of congestive heart failure, cirrhosis, or kidney failure. Diuretic use may increase the risk. If you are pregnant or nursing a baby, seek the advice of a health professional before using this product.” It meets the requirements for Labeling under Botanical Extracts 565.
USP Reference standards 11
USP Glycyrrhizic Acid RS.
Thin-layer chromatographic identification test 201
Standard solution, Application volume, Developing solvent system, and Procedure— Proceed as directed for Thin-layer chromatographic identification test under Licorice.
Test solution— Dissolve about 0.6 g of Powdered Extract, accurately weighed, in 10 mL of a mixture of alcohol and water (1:1).
Loss on drying 731 Dry it at 105 for 6 hours: it loses not more than 10.0% of its weight.
Total ash 561: not more than 12.0%.
Pesticide residues 561: meets the requirements.
Content of glycyrrhizic acid—
Solvent, Mobile phase, Standard solution, and Chromatographic system— Proceed as directed for Content of glycyrrhizic acid under Licorice.
Test solution— Transfer about 150 mg of Powdered Extract, accurately weighed, to a suitable flask, add 25 mL of Solvent, heat at 50 for 30 minutes, cool, centrifuge, and decant the supernatant into a 100-mL volumetric flask. Mix the residue with 20 mL of Solvent, repeat the above procedure, and dilute with Solvent to volume.
Procedure— Proceed as directed for Content of glycyrrhizic acid under Licorice, except to calculate the percentage of glycyrrhizic acid (C42H62O16) in the portion of Powdered Extract taken by the formula:
10,000(C/W)(rU / rS)
in which W is the weight, in mg, of the Powdered Extract taken to prepare the Test solution; and the other terms are as defined therein.
Auxiliary Information— Please check for your question in the FAQs before contacting USP.
Topic/Question Contact Expert Committee
Monograph Maged H. Sharaf, Ph.D.
Senior Scientist
(DSB05) Dietary Supplements - Botanicals
Reference Standards Lili Wang, Technical Services Scientist
USP32–NF27 Page 1042
Pharmacopeial Forum: Volume No. 30(2) Page 574
Chromatographic Column—
Chromatographic columns text is not derived from, and not part of, USP 32 or NF 27.