A: Transfer 30 mg of Hyoscyamine Hydrobromide and 36 mg of USP Hyoscyamine Sulfate RS to individual 60-mL separators with the aid of 5-mL portions of water. To each separator add 1.5 mL of 1 N sodium hydroxide and 10 mL of chloroform. Shake for 1 minute, allow the layers to separate, and filter the chloroform extracts through separate filters of about 2 g of anhydrous granular sodium sulfate supported on pledgets of glass wool. Extract each aqueous layer with two additional 10-mL portions of chloroform, filtering and combining with the respective main extracts. Evaporate the chloroform solutions under reduced pressure to dryness, and dissolve each residue in 10 mL of carbon disulfide: the IR absorption spectrum, determined in a 1-mm cell, of the solution obtained from the test specimen exhibits maxima only at the same wavelengths as that of the solution obtained from the Reference Standard.

B: To about 1 mL of a solution (1 in 20) add gold chloride TS, dropwise with shaking, until a definite precipitate separates. Add a small amount of 3 N hydrochloric acid, dissolve the precipitate with the aid of heat, and allow the solution to cool: lustrous reddish brown scales that may be accompanied by reddish brown needles are formed (distinction from atropine and scopolamine).

C: To an aqueous solution (1 in 20) add silver nitrate TS: a yellowish-white precipitate is formed, and it is insoluble in nitric acid.

Test solution: 50 mg per mL, in water.