Gramicidin
Gramicidin.
Gramicidin
[1405-97-6].» Gramicidin is an antibacterial substance produced by the growth of Bacillus brevis Dubos (Fam. Bacillaceae). It may be obtained from tyrothricin. It has a potency of not less than 900 µg of gramicidin per mg, calculated on the dried basis.
Packaging and storage—
Preserve in tight containers.
Melting temperature, Class Ia 
741
:
not lower than 229
, determined after drying.
741:
not lower than 229, determined after drying.
Crystallinity 695:
meets the requirements.
695:
meets the requirements.
Loss on drying 731—
Dry about 100 mg in a capillary-stoppered bottle in vacuum at 60 for 3 hours: it loses not more than 3.0% of its weight.
731—
Dry about 100 mg in a capillary-stoppered bottle in vacuum at 60 for 3 hours: it loses not more than 3.0% of its weight.
Residue on ignition 281:
not more than 1.0%, the charred residue being moistened with 2 mL of nitric acid and 5 drops of sulfuric acid.
281:
not more than 1.0%, the charred residue being moistened with 2 mL of nitric acid and 5 drops of sulfuric acid.
Assay—
Proceed with Gramicidin as directed under Antibiotics—Microbial Assays 81.
81.
Auxiliary Information—
Please check for your question in the FAQs before contacting USP.
| Topic/Question | Contact | Expert Committee |
| Monograph | Ahalya Wise, M.S.
Scientist 1-301-816-8161 |
(MDANT05) Monograph Development-Antibiotics |
| Reference Standards | Lili Wang, Technical Services Scientist 1-301-816-8129 RSTech@usp.org |
USP32–NF27 Page 2528