Test solution— Transfer about 100 mg of Powdered Extract, accurately weighed, to a 10-mL volumetric flask, and dilute with a mixture of methanol and water (1:1) to volume. Sonicate for 20 minutes, cool to room temperature, mix, and filter.

Standard solution, Application volume, Developing solvent system, and Procedure— Proceed as directed for Thin-layer chromatographic identification test under Goldenseal.

Mobile phase, Standard solution, and Chromatographic system— Proceed as directed for Content of berberine and hydrastine and limit of palmatine under Goldenseal.

Test solution— Transfer about 100 mg of Powdered Extract, accurately weighed, to a 50-mL volumetric flask, and dilute with a mixture of water and methanol (1:1). Sonicate for 20 minutes, cool to room temperature, mix, and filter.

Procedure— Proceed as directed for Content of berberine and hydrastine and limit of palmatine under Goldenseal, except to calculate the percentages of hydrastine and berberine in the portion of Powdered Extract taken by the formula: in which W is the weight, in mg, of Powdered Extract taken for the Test solution; and the other terms are as defined therein: not less than 5% of hydrastine is found; and not less than 10% of hydrastine plus berberine is found. Using the values obtained from the chromatogram of the Test solution, divide the peak area of berberine by the peak area of any peak at the locus for palmatine (if present): the ratio is more than 50:1. [note—The sample to be used in this test should not be subjected to the conditions specified in the test for Loss on drying. A separate sample is used to determine the content on the dried basis.]