A: Thin-Layer Chromatographic Identification Test 201—

B: The retention times of the peaks for ginsenosides Rg1, Re, Rb1, Rb2, Rc, and Rd in the chromatogram of the Test solution correspond to those in the chromatogram of Standard solution 1, as obtained in the test for Content of ginsenosides. The ratio of the peak response for Rb2 to the peak response for Rb1 is less than 0.4, and the ratio of the peak response for Rg1 to the peak response for Rb1 is less than 0.3. The chromatogram of the Test solution shows no significant peak at the retention time corresponding to that for ginsenoside Rf in the chromatogram of Standard solution 2, as obtained in the test for Content of ginsenosides.

Solution A, Solution B, Mobile phase, Standard solution 1, Standard solution 2, and Chromatographic system— Proceed as directed for Content of ginsenosides under American Ginseng.

Test solution— Transfer a quantity of Powdered Extract, equivalent to 5 mg of ginsenosides, accurately weighed, to a suitable container. Dissolve in 10.0 mL of a mixture of water and alcohol (6:4) sonicating for 10 minutes, and filter.

Procedure— Proceed as directed for Content of ginsenosides under American Ginseng. Calculate the percentages of individual ginsenosides in the portion of Powdered Extract taken by the formula: in which C is the concentration, in mg per mL, of ginsenoside Rg1, Re, Rb1, Rc, Rb2, or Rd in the appropriate Standard solution; W is the weight, in mg, of Powdered Extract taken to prepare the Test solution; and rU and rS are the peak responses of ginsenoside Rg1, Re, Rb1, Rc, Rb2, or Rd obtained from the Test solution and the appropriate Standard solution, respectively. Calculate the percentage of total ginsenosides in the portion of Powdered Extract taken by adding the individual percentages.