A: Proceed as directed in the test for Related compounds under Amitraz, except to prepare a test solution by diluting the Concentrate with toluene to obtain a solution containing about 5 mg of amitraz per mL and to prepare a Standard solution of USP Amitraz RS in toluene containing 5 mg per mL: the RF value of the principal spot in the chromatogram obtained from the test solution corresponds to that in the chromatogram obtained from the Standard solution.

B: The retention time of the major peak in the chromatogram of the Reference solution corresponds to that in the chromatogram of the Standard preparation, as obtained in the Assay.

Internal standard solution, Standard preparation, and Chromatographic system— Proceed as directed in the Assay under Amitraz.

Assay preparation— Transfer an accurately measured volume of Concentrate, equivalent to about 80 mg of amitraz, to a 10-mL volumetric flask, dilute with Internal standard solution to volume, and mix.

Reference solution— Transfer an accurately measured volume of Concentrate, equivalent to about 80 mg of amitraz, to a 10-mL volumetric flask, dilute with methyl acetate to volume, and mix.

Procedure— Proceed as directed for Procedure in the Assay under Amitraz. Calculate the quantity, in mg, of amitraz (C19H23N3) in each mL of the Concentrate taken by the formula: in which V is the volume, in mL, of Concentrate taken to prepare the Assay preparation; and the other terms are as defined therein.