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Powdered Turmeric Extract
» Powdered Turmeric Extract is prepared from the pulverized rhizomes of Curcuma longa L. (Fam. Zingiberaceae), using acetone, methanol, or other suitable solvents. It contains not less than 20 percent of total curcuminoids, calculated on the dried basis. It may contain other added substances.
Packaging and storage Preserve in well-closed containers, protected from light and moisture, and store at controlled room temperature.
Labeling The label states the Latin binominal and, following the official name, the part of the plant contained in the article. It meets other labeling requirements under Botanical Extracts 565.
USP Reference standards 11
USP Bisdesmethoxycurcumin RS.
USP Curcumin RS.
USP Curcuminoids RS.
USP Desmethoxycurcumin RS.
A: Thin-Layer Chromatographic Identification Test 201
Test solution: 10 mg per mL of Extract in acetone.
Standard solution, Application volume, Developing solvent system, and Procedure Proceed as directed for Identification test A under Turmeric.
B: The retention times of the peaks for curcumin, desmethoxycurcumin, and bisdesmethoxycurcumin in the chromatogram of the Test solution correspond to those in the chromatogram of the Standard solution for the appropriate USP Reference Standard, as obtained in the test for Content of curcuminoids.
Microbial enumeration 2021 The total aerobic microbial count does not exceed 104 cfu per g. The total combined yeast and mold count does not exceed 103 cfu per g.
Absence of specified microorganisms 2022 It meets the requirements of the tests for absence of Salmonella species and Escherichia coli.
Loss on drying 731 Dry 1.0 g at 105 for 2 hours: it loses not more than 7.0% of its weight.
Aflatoxins 561: meets the requirements.
Pesticide residues 561: meets the requirements.
Heavy metals, Method III 231: not more than 20 µg per g.
Content of curcuminoids
Mobile phase, Standard solution 1, Standard solution 2, Standard solution 3, Standard solution 4, and Chromatographic system Proceed as directed in the test for Content of curcuminoids under Turmeric.
Test solution Transfer about 100 mg of Extract, accurately weighed, to a 50-mL volumetric flask, add about 30 mL of acetone, and sonicate for 30 minutes. Dilute with acetone to volume, mix, and centrifuge. Transfer 5 mL of the supernatant to a 50-mL volumetric flask, dilute with Mobile phase to volume, mix, and before injection pass through a filter having a 0.45-µm porosity.
Procedure Proceed as directed in the test for Content of curcuminoids under Turmeric. Separately calculate the percentages of curcumin, desmethoxycurcumin, and bisdesmethoxycurcumin in the portion of Extract taken by the formula:
50,000(C / W)(rU / rS)in which C is the concentration, in mg per mL, of USP Curcumin RS, USP Desmethoxycurcumin RS, or USP Bisdesmethoxycurcumin RS in the corresponding Standard solution; W is the weight, in mg, of Extract taken to prepare the Test solution; rU is the peak response of the relevant analyte in the Test solution; and rS is the peak response of the relevant analyte in the corresponding Standard solution. Add the percentages due to curcumin, desmethoxycurcumin, and bisdesmethoxycurcumin: not less than 20% is found.
Other requirements It meets the requirements of the test for Residual Solvents under Botanical Extracts 565.USP32
Auxiliary Information Please check for your question in the FAQs before contacting USP.Chromatographic Column
USP32NF27 Page 1079Pharmacopeial Forum: Volume No. 33(6) Page 1232
Chromatographic columns text is not derived from, and not part of, USP 32 or NF 27.