(B + G)/(D + F)

Solvent, Solution A, Solution B, Mobile phase, Standard solution 1, Standard solution 2, and Chromatographic system— Proceed as directed in the test for Content of isoflavones under Red Clover.

Test solution— Accurately weigh not fewer than 20 Tablets, and pulverize, using a mortar and pestle. Transfer an accurately weighed quantity of the powder, equivalent to 40 mg of the labeled amount of isoflavones, to a 250-mL volumetric flask. Add 15 mL of water, shake to disperse the powder, add 15 mL of dehydrated alcohol and about 200 mL of Solvent, and sonicate for 30 minutes. If dark particles are present in the bottom of the flask, sonicate again for an additional 10 minutes or until they disappear. Cool to room temperature, and dilute with Solvent to volume. Transfer 50.0 mL of the resulting solution to a round-bottom flask, and evaporate to dryness under vacuum. Add 15 mL of 2 N hydrochloric acid, and heat in a water bath for 30 minutes. Quantitatively transfer this solution with the aid of about 15 mL of alcohol, to a 50-mL volumetric flask, and dilute with Solvent to volume. Pass 5 mL of the solution through a filter having a 0.45-µm porosity, discarding the first 4 mL of the filtrate. Collect the remaining 1 mL of filtrate for testing.

Procedure— Proceed as directed in the test for Content of isoflavones under Red Clover. Calculate the content of isoflavones, in mg, in the portion of Tablets taken by the formula: in which the terms are as defined therein.