Aromatic Ammonia Solution

Sal Volatile Solution

Ammonium Bicarbonate	25 g
Nutmeg Oil	0.3 mL
Lemon Oil	0.5 mL
Ethanol (90 per cent)	37.5 mL
Strong Ammonia Solution	67.5 mL
Purified Water, freshly boiled and cooled	Sufficient to produce 1000 mL

The following directions apply.

Dissolve the Ammonium Bicarbonate in 800 mL of the Purified Water. Separately dissolve the Lemon Oil and the Nutmeg Oil in the Ethanol (90 per cent). Add the ethanolic solution to the aqueous solution and add the Strong Ammonia Solution and sufficient Purified Water to produce 1000 mL. Add 25 g of previously sterilised Purified Talc, shake, allow to stand for a few hours, shaking occasionally, and filter.

1.12 to 1.25% w/v.

2.76 to 3.24% w/v.

2.6 to 3.5% v/v when determined by the following method.

Carry out the method for gas chromatography, Appendix III B, using the following solutions:

(1) 1.5% v/v of absolute ethanol and 1.5% v/v of propan-1-ol (internal standard).

(2) Dilute a volume of the preparation being examined with water to contain between 1.0% and 2.0% v/v of ethanol.

(3) Prepare solution in the same manner as solution (2) but adding sufficient of the internal standard to produce a final concentration of 1.5% v/v.

(a) Use a column (1.5 m × 4 mm) packed with porous polymer beads (100 to 120 mesh) (Porapak Q and Chromosorb 101 are suitable).

(b) Use helium as the carrier gas at 1.7 mL per minute.

(c) Use isothermal conditions maintained at 150°.

(d) Use an inlet temperature of 170°.

(e) Use a flame ionisation detector at a temperature of 170°.

(f) Inject 1 µL of each solution.

Calculate the percentage content of ethanol in the solution from the areas of the peaks due to ethanol in the chromatograms obtained with solutions (1) and (3).

0.980 to 1.005 g, Appendix V G.

To 20 mL add 50 mL of 1m hydrochloric acid VS, boil, cool and titrate the excess of acid with 1m sodium hydroxide VS using methyl red solution as indicator. Each mL of 1m hydrochloric acid VS, after subtraction of the volume of 1m sodium hydroxide VS required in the Assay for ammonium carbonate, is equivalent to 17.03 mg of NH₃.

To 20 mL add 25 mL of 1m sodium hydroxide VS and 40 mL of barium chloride solution, heat on a water bath for 15 minutes, cool, add 10 mL of formaldehyde solution previously neutralised to thymol blue solution and titrate the excess of alkali with 1m hydrochloric acid VS, using thymol blue solution as indicator, to the grey colour indicative of pH 8.8. Each mL of 1m sodium hydroxide VS is equivalent to 48.04 mg of (NH₄)₂CO₃.