Tris-chloride Buffer pH 7.4

Tris-chloride Buffer pH 7.4 Tris(hydroxymethyl)aminomethane sodium chloride buffer solution pH 7.4

Dissolve 6.08 g of tris(hydroxymethyl)aminomethane R, 8.77 g of sodium chloride R in 500 mL of distilled water R. Add 10.0 g of bovine albumin R. Adjust the pH (2.2.3) using hydrochloric acid R. Dilute to 1000.0 mL with distilled water R.