Strong Glutaraldehyde Solution

C₅H₈O₂    100.1    111-30-8

        (anhydrous)

Used in treatment of warts.

Glutaraldehyde Solution

Strong Glutaraldehyde Solution is an aqueous solution of glutaraldehyde (pentanedial). It contains not less than 47.0% and not more than 53.0% w/w of glutaraldehyde, C₅H₈O₂.

A colourless or almost colourless solution.

A.  Heat 1  ml with 10  ml of a solution containing 1  g of hydroxylamine hydrochloride and 2  g of sodium acetate in water on a water bath for 10  minutes, allow to cool and filter. The melting point of the residue, after washing with water and drying at 105°, is about 178°, Appendix V A.

B.  Add 0.05  ml to 2  ml of ammoniacal silver nitrate solution and mix gently for a few minutes. Silver is deposited.

Dilute 10  ml with 10  ml of carbon dioxide-free water and titrate with 0.1m sodium hydroxide VS using bromothymol blue solution R3 as indicator. Not more than 5.0  ml of 0.1m sodium hydroxide VS is required to change the colour of the solution.

Dilute 1  volume with 4  volumes of water. The resulting solution is clear, Appendix IV A, and not more intensely coloured than reference solution B₆, Appendix IV B, Method I.

1.126  to 1.134  g, Appendix V G.

Dissolve 4  g in 100  ml of a 7% w/v solution of hydroxylamine hydrochloride previously neutralised to bromophenol blue solution with 1m sodium hydroxide VS and allow to stand for 30  minutes. Add 20  ml of petroleum spirit (boiling range, 40° to 60°) and titrate with 1m sodium hydroxide VS until the colour of the aqueous phase matches that of a 7% w/v solution of hydroxylamine hydrochloride previously neutralised to bromophenol blue solution with 1m sodium hydroxide VS. Each ml of 1m sodium hydroxide VS is equivalent to 50.05  mg of C₅H₈O₂.

Strong Glutaraldehyde Solution should be stored at a temperature not exceeding 15°.