Plasma, Platelet-poor

Plasma, Platelet-poor Withdraw 45  ml of human blood into a 50  ml plastic syringe containing 5  ml of a sterile 3.8% w/v solution of sodium citrate . Immediately centrifuge the citrated whole blood at 1150  g for 30 minutes at 4°. Remove the upper two thirds of the supernatant plasma using a plastic syringe and immediately centrifuge at 3500  g for 30 minutes at 4°. Remove the upper two thirds of the liquid and freeze it rapidly in suitable quantities in plastic tubes at a temperature of –40° or below. Use plastic or siliconised equipment throughout.